PMID- 31169343 OWN - NLM STAT- MEDLINE DCOM- 20200626 LR - 20200626 IS - 1615-9861 (Electronic) IS - 1615-9853 (Linking) VI - 19 IP - 21-22 DP - 2019 Nov TI - Identification of miR-145-5p-Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma. PG - e1900020 LID - 10.1002/pmic.201900020 [doi] AB - This study intends to investigate the transcriptional regulatory role of miR-145-5p in laryngeal squamous cell carcinoma (LSCC). LSCC cell line TU-177 is transfected with miR-145-5p mimics, generating miR-145-5p-overexpression LSCC cells. Whole transcriptome microarrays are used to investigate the differentially expressed lncRNAs, circRNAs, mRNAs, and miRNAs. The target genes of miRNAs are predicted and performed functional annotation. Additionally, the circRNAs, lncRNAs, and mRNAs that interact with miRNAs are predicted, and then the competing endogenous RNAs (ceRNAs) are predicted. Microarray analysis identifies 26 miRNAs, 248 mRNAs, 1118 lncRNAs, and 382 circRNAs differentially expressed in miR-145-5p overexpressed LSCC cells. Overall, 675 target genes are identified for the differentially expressed miRNAs, which involved in cell adhesion associated gene ontology (GO) terms, and MAPK and FoxO signaling pathways. The up-regulated mRNAs involved in the pathway of ABC transporters, while the down-regulated mRNAs involved in pathway of olfactory transduction. Moreover, 149 ceRNAs are predicted, which are associated with apoptosis, Wnt pathway, and metabolic pathway. Furthermore, qPCR results confirm that miR-145-5p affects expression of lncRNAs, miRNAs, mRNAs, and circRNAs in LSCC cells. Collectively, miR-145-5p may be inhibits LSCC progression via ceRNA-mediated pathways, such as WNT2B-miR-145-5p-NONHSAT127539.2, CASP10-miR-145-5p-NONHSAT127539.2, CASP10-miR-145-5p-circ_0003519, and TPO-miR-145-5p-circ_0003519. CI - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. FAU - Gao, Wei AU - Gao W AUID- ORCID: 0000-0001-7836-2851 AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Zhang, Yuliang AU - Zhang Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Niu, Min AU - Niu M AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Bo, Yunfeng AU - Bo Y AD - Department of Pathology, Shanxi Cancer Hospital, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. FAU - Li, Huizheng AU - Li H AD - Department of Otolaryngology Head and Neck Surgery, Dalian Municipal Friendship Hospital, Dalian, 116001, Liaoning, P. R. China. FAU - Xue, Xuting AU - Xue X AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Lu, Yan AU - Lu Y AD - Department of Otolaryngology Head and Neck Surgery, The First Hospital, Jinzhou Medical University, Jinzhou, 121001, Liaoning, P. R. China. FAU - Zheng, Xiwang AU - Zheng X AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Tang, Yemei AU - Tang Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Cui, Jiajia AU - Cui J AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - He, Long AU - He L AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Thorne, Rick F AU - Thorne RF AD - Translational Research Institute, Henan Provincial People's Hospital, School of Medicine, Henan University, Zhengzhou, 450053, Henan, P. R. China. AD - School of Environmental and Life Sciences, The University of Newcastle, Callaghan, 2308, NSW, Australia. FAU - Wang, Binquan AU - Wang B AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. FAU - Wu, Yongyan AU - Wu Y AUID- ORCID: 0000-0003-1669-3860 AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - Otolaryngology Head and Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, 030001, Shanxi, P. R. China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, P. R. China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190620 PL - Germany TA - Proteomics JT - Proteomics JID - 101092707 RN - 0 (MIRN145 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Messenger) SB - IM MH - Carcinoma, Squamous Cell/*genetics MH - Cell Line, Tumor MH - Gene Expression Profiling MH - Gene Expression Regulation, Neoplastic MH - *Gene Regulatory Networks MH - Humans MH - Laryngeal Neoplasms/*genetics MH - MicroRNAs/genetics/*metabolism MH - Molecular Sequence Annotation MH - RNA, Messenger/genetics/metabolism OTO - NOTNLM OT - apoptosis OT - competing endogenous RNA OT - laryngeal squamous cell carcinoma OT - metabolic reprogramming OT - miR-145 EDAT- 2019/06/07 06:00 MHDA- 2020/06/27 06:00 CRDT- 2019/06/07 06:00 PHST- 2019/01/13 00:00 [received] PHST- 2019/05/02 00:00 [revised] PHST- 2019/06/07 06:00 [pubmed] PHST- 2020/06/27 06:00 [medline] PHST- 2019/06/07 06:00 [entrez] AID - 10.1002/pmic.201900020 [doi] PST - ppublish SO - Proteomics. 2019 Nov;19(21-22):e1900020. doi: 10.1002/pmic.201900020. Epub 2019 Jun 20.