PMID- 31170640
OWN - NLM
STAT- MEDLINE
DCOM- 20190906
LR  - 20200930
IS  - 1873-2690 (Electronic)
IS  - 0981-9428 (Linking)
VI  - 141
DP  - 2019 Aug
TI  - Response of sugar metabolism in apple leaves subjected to short-term drought 
      stress.
PG  - 164-171
LID - S0981-9428(19)30225-6 [pii]
LID - 10.1016/j.plaphy.2019.05.025 [doi]
AB  - For a comprehensive understanding of gene expression, enzyme activity and sugar 
      concentrations in response to short-term water deficit in apple (Greensleeves), 
      sugar-modulated gene expression and enzyme activities were analyzed. Water stress 
      resulted in the accumulation of sorbitol, glucose, fructose, galactose and 
      starch, accompanied by a significant reduction in photosynthesis and sucrose 
      concentration. In response to short-term water deficits, the activities of 
      aldose-6-phosphate reductase (A6PR; EC 1.1.1.200), sorbitol dehydrogenase (SDH; 
      EC 1.1.1.14), neutral invertase (NINV; EC 3.2.1.26), sucrose synthase (SUSY; EC 
      2.4.1.13), and fructokinase (FK; EC 2.7.1.4) were higher, whereas cell wall 
      invertase (CWINV; EC 3.2.1.26) and hexokinase (HK; EC 2.7.1.1) activities were 
      lower. In addition, sucrose phosphate synthase (SPS; EC 2.4.1.14) activity 
      increased during the initial stages of dehydration and then decreased as the 
      drought strengthened. Transcript levels of MdA6PR, MdSDH1/2, MdNINV1/2, MdSUSY3, 
      MdFK1/2/4, MdSOT1/2, MdSUC1-3, MdTMT2/3, MdvGT1, MdpGlcT1-4 were upregulated, 
      whereas transcript levels of MdCWINV1/2, MdHK1/2/3/5, and MdTMT1 were 
      downregulated after 6 days of water stress. These findings suggest that the 
      sorbitol metabolism pathway is induced and high levels of hexose derived from 
      photosynthetic products are transported into vacuoles for adjustment to the water 
      deficit. Our results provide insights into the relationships between sugar levels 
      and sugar-modulated gene and enzyme activity in response to the imposition of 
      short-term water stress.
CI  - Copyright (c) 2019 Elsevier Masson SAS. All rights reserved.
FAU - Yang, Jingjing
AU  - Yang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Li, Chuang
AU  - Li C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Zhang, Zhao
AU  - Zhang Z
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Ma, Fengwang
AU  - Ma F
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Li, Mingjun
AU  - Li M
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory 
      of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China. Electronic address: limingjun@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190531
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 0 (Plant Proteins)
RN  - 506T60A25R (Sorbitol)
RN  - 57-50-1 (Sucrose)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - EC 2.4.1.13 (sucrose synthase)
RN  - EC 2.4.1.14 (sucrose-phosphate synthase)
RN  - EC 3.2.1.26 (beta-Fructofuranosidase)
SB  - IM
MH  - Carbohydrate Metabolism/*genetics
MH  - Cell Wall/metabolism
MH  - *Droughts
MH  - Fruit/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Glucosyltransferases/metabolism
MH  - Malus/genetics/*physiology
MH  - Photosynthesis
MH  - Plant Leaves/*physiology
MH  - Plant Proteins/metabolism
MH  - Plants, Genetically Modified/physiology
MH  - Sorbitol/metabolism
MH  - Stress, Physiological
MH  - Sucrose/metabolism
MH  - beta-Fructofuranosidase/metabolism
OTO - NOTNLM
OT  - Drought
OT  - Enzyme
OT  - Expression
OT  - Hexose
OT  - Sorbitol
OT  - Sucrose
OT  - Sugar
EDAT- 2019/06/07 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/07 06:00
PHST- 2018/12/22 00:00 [received]
PHST- 2019/05/24 00:00 [revised]
PHST- 2019/05/27 00:00 [accepted]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2019/06/07 06:00 [entrez]
AID - S0981-9428(19)30225-6 [pii]
AID - 10.1016/j.plaphy.2019.05.025 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2019 Aug;141:164-171. doi: 10.1016/j.plaphy.2019.05.025. 
      Epub 2019 May 31.