PMID- 31171270
OWN - NLM
STAT- MEDLINE
DCOM- 20200106
LR  - 20200106
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 17
IP  - 5
DP  - 2019 May 20
TI  - Modified Da-chai-hu Decoction regulates the expression of occludin and NF-kappaB to 
      alleviate organ injury in severe acute pancreatitis rats.
PG  - 355-362
LID - S1875-5364(19)30041-X [pii]
LID - 10.1016/S1875-5364(19)30041-X [doi]
AB  - Modified Da-chai-hu Decoction (MDD), a traditional Chinese medicinal formulation, 
      which was empirically generated from Da-chai-hu decoction, has been utilized to 
      treat severe acute pancreatitis (SAP) for decades. The aim of the present study 
      was to explore its potential organprotective mechanism in SAP. In the present 
      study, rat SAP model was induced by retrograde injection of 3.5% sodium 
      taurocholate into the biliopancreatic duct, MDD (23.35 g/kg body weight, twelve 
      times the clinical dose) were orally given at 2 h before and 10 h after 
      injection. At 12 h after model induction, blood was taken from vena cava for 
      analysis of amylase, diamine oxidase (DAO), pulmonary surfactant protein-A 
      (SP-A), and C-reactive protein (CRP). Histopathological change of pancreas, ileum 
      and lung was assayed by H&E staining, myeloperoxidase (MPO) activity were 
      determinated using colorimetric assay, and the expressions of occludin and 
      nuclear factor-kappaB (NF-kappaB) were detected by real-time RT-PCR and western blot, 
      respectively. In addition, the tissue concentrations of tumor necrosis factor-alpha 
      (TNF-alpha), interleukin-1beta (IL-1beta), and monocyte chemoattractant protein-1 (MCP-1) 
      were measured by enzyme-linked immunosorbent assay (ELISA). The results showed 
      that in SAP rats, MDD significantly alleviated histopathological damage, 
      depressed the MPO activity and the concentrations of TNF-alpha, IL-1beta, and MCP-1 of 
      pancreas, ileum and lung, and reduced the serum levels of amylase [(3283.4 +/- 
      585.5) U.L(-1)vs (5626.4 +/- 795.1)U.L(-1)], DAO [(1100.1 +/- 334.3) U.L(-1)vs 
      (1666.4 +/- 525.3) U.L(-1)] and CRP [(7.6 +/- 1.2) mug.mL(-1)vs (17.8 +/- 3.8) 
      mug.mL(-1)]. However, the serum SP-A concentration [(106.1 +/- 16.6) pg.mL(-1)vs 
      (90.1 +/- 14.9) pg.mL(-1)] was elevated when treated SAP rats with MDD. 
      Furthermore, MDD increased the occludin expression and reduced the NF-kappaB 
      expression in pancreas, ileum and lung of SAP rats. Our findings suggested that 
      MDD administration was an effective therapeutic approach for SAP treatment. It 
      could up-regulate occludin expression to protect intercellular tight junction and 
      down-regulate NF-kappaB expression to inhibit inflammatory reaction of pancreas, 
      ileum and lung.
CI  - Copyright (c) 2019 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Zhao, Guang
AU  - Zhao G
AD  - Hepatobiliary and Pancreatic Surgery, Tianjin Nankai Hospital, Tianjin Hospital 
      of Integrated Traditional Chinese and Western medicine, Tianjin 300100, China; 
      Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China.
FAU - Zhuo, Yu-Zhen
AU  - Zhuo YZ
AD  - Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China; 
      Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China.
FAU - Cui, Li-Hua
AU  - Cui LH
AD  - Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China; 
      Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China.
FAU - Li, Cai-Xia
AU  - Li CX
AD  - Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China; 
      Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China.
FAU - Chen, Sha-Yan
AU  - Chen SY
AD  - Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China; 
      Department of Clinical Laboratory, Tianjin Nankai Hospital, Tianjin Hospital of 
      Integrated Traditional Chinese and Western medicine, Tianjin 300100, China.
FAU - Li, Dan
AU  - Li D
AD  - Hepatobiliary and Pancreatic Surgery, Tianjin Nankai Hospital, Tianjin Hospital 
      of Integrated Traditional Chinese and Western medicine, Tianjin 300100, China; 
      Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China.
FAU - Liu, Jun-Hong
AU  - Liu JH
AD  - Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China.
FAU - Li, Di-Hua
AU  - Li DH
AD  - Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China.
FAU - Cui, Nai-Qiang
AU  - Cui NQ
AD  - Hepatobiliary and Pancreatic Surgery, Tianjin Nankai Hospital, Tianjin Hospital 
      of Integrated Traditional Chinese and Western medicine, Tianjin 300100, China; 
      Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China.
FAU - Zhang, Shu-Kun
AU  - Zhang SK
AD  - Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China; 
      Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai 
      Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western 
      medicine, Tianjin 300100, China. Electronic address: shu1971@163.com.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (Biomarkers)
RN  - 0 (Bupleurum root extract)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, rat)
RN  - 0 (Plant Extracts)
RN  - 5E090O0G3Z (Taurocholic Acid)
SB  - IM
MH  - Animals
MH  - Biomarkers/blood/metabolism
MH  - Bupleurum
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Ileum/drug effects/metabolism/pathology
MH  - Lung/drug effects/metabolism/pathology
MH  - Male
MH  - NF-kappa B/genetics/*metabolism
MH  - Occludin/genetics/*metabolism
MH  - Pancreas/drug effects/metabolism/pathology
MH  - Pancreatitis, Acute Necrotizing/chemically induced/*drug therapy/*pathology
MH  - Plant Extracts/*therapeutic use
MH  - Rats, Wistar
MH  - Taurocholic Acid/toxicity
OTO - NOTNLM
OT  - Ileum
OT  - Lung
OT  - Modified Da-chai-hu Decoction
OT  - Nuclear factor-kappaB
OT  - Occludin
OT  - Severe acute pancreatitis
EDAT- 2019/06/07 06:00
MHDA- 2020/01/07 06:00
CRDT- 2019/06/08 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/06/08 06:00 [entrez]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
AID - S1875-5364(19)30041-X [pii]
AID - 10.1016/S1875-5364(19)30041-X [doi]
PST - ppublish
SO  - Chin J Nat Med. 2019 May 20;17(5):355-362. doi: 10.1016/S1875-5364(19)30041-X.