PMID- 31173197
OWN - NLM
STAT- MEDLINE
DCOM- 20191223
LR  - 20200225
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 44
IP  - 2
DP  - 2019 Aug
TI  - Protective effects of salvianolic acid B against hydrogen peroxide‑induced 
      apoptosis of human umbilical vein endothelial cells and underlying mechanisms.
PG  - 457-468
LID - 10.3892/ijmm.2019.4227 [doi]
AB  - Salvianolic acid B (Sal B) is a water‑soluble active component of Danshen and has 
      anti‑atherosclerotic effects. The present study aimed to evaluate the 
      cytoprotective effects of Sal B against hydrogen peroxide (H2O2)‑induced 
      oxidative stress damage in human umbilical vein endothelial cells (HUVECs) and 
      investigate the underlying mechanisms. It was revealed that Sal B protected the 
      cells from H2O2‑induced damage, as indicated by MTT results showing enhanced cell 
      viability and by flow cytometric analysis showing reduced apoptosis of cells 
      challenged with H2O2. Furthermore, as an underlying mechanism, the enhancement of 
      autophagy was indicated to be accountable for the decrease in apoptosis, as Sal B 
      caused the upregulation of light chain 3‑Ⅱ and Beclin‑1, and downregulation of 
      p62 under H2O2‑induced oxidative stress. Finally, Sal B increased the 
      phosphorylation of AMP kinase (AMPK) and decreased the phosphorylation of 
      mammalian target of rapamycin (mTOR), but had no effect on the phosphorylation of 
      AKT. In conclusion, the present study revealed that Sal B protects HUVECs from 
      oxidative stress, at least partially by promoting autophagy via activation of the 
      AMPK pathway and downregulation of the mTOR pathway.
FAU - Gao, Shan
AU  - Gao S
AD  - College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. 
      China.
FAU - Li, Shiqin
AU  - Li S
AD  - College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. 
      China.
FAU - Li, Qin
AU  - Li Q
AD  - Department of Infectious Gastroenterology, Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
FAU - Zhang, Fuyong
AU  - Zhang F
AD  - Department of Pharmacy, People's Hospital of Deyang City, Deyang, Sichuan 618000, 
      P.R. China.
FAU - Sun, Mengqi
AU  - Sun M
AD  - College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. 
      China.
FAU - Wan, Zilin
AU  - Wan Z
AD  - College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. 
      China.
FAU - Wang, Shurong
AU  - Wang S
AD  - Department of Pharmacy, Affiliated Hospital of Southwest Medical University, 
      Luzhou, Sichuan 646000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190531
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Antioxidants)
RN  - 0 (Benzofurans)
RN  - 0 (Drugs, Chinese Herbal)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - C1GQ844199 (salvianolic acid B)
SB  - IM
MH  - Antioxidants/chemistry/*pharmacology
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Benzofurans/chemistry/*pharmacology
MH  - Cytoprotection/drug effects
MH  - Drugs, Chinese Herbal/chemistry/pharmacology
MH  - Endothelial Cells/cytology/*drug effects/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hydrogen Peroxide/*metabolism
MH  - Oxidative Stress/drug effects
MH  - Salvia miltiorrhiza/chemistry
PMC - PMC6605496
EDAT- 2019/06/08 06:00
MHDA- 2019/12/24 06:00
PMCR- 2019/05/31
CRDT- 2019/06/08 06:00
PHST- 2018/11/27 00:00 [received]
PHST- 2019/05/23 00:00 [accepted]
PHST- 2019/06/08 06:00 [pubmed]
PHST- 2019/12/24 06:00 [medline]
PHST- 2019/06/08 06:00 [entrez]
PHST- 2019/05/31 00:00 [pmc-release]
AID - ijmm-44-02-0457 [pii]
AID - 10.3892/ijmm.2019.4227 [doi]
PST - ppublish
SO  - Int J Mol Med. 2019 Aug;44(2):457-468. doi: 10.3892/ijmm.2019.4227. Epub 2019 May 
      31.