PMID- 31175668
OWN - NLM
STAT- MEDLINE
DCOM- 20201120
LR  - 20220531
IS  - 1365-2842 (Electronic)
IS  - 0305-182X (Linking)
VI  - 47 Suppl 1
DP  - 2020 Nov
TI  - TGF-beta1-induced connexin43 promotes scar formation via the Erk/MMP-1/collagen III 
      pathway.
PG  - 99-106
LID - 10.1111/joor.12829 [doi]
AB  - Wound healing can be divided into different phases, and timely initiation and 
      cessation of these stages is key to successful wound healing; otherwise, scar 
      tissue forms in the wounded area. Connexins (Cxs) were confirmed to influence 
      scar formation, and Cx43, an indispensable member of the Cx family, was shown to 
      be involved in this process. Our study investigated the regulatory role of Cx43 
      in scar formation and the possible cell signalling pathways. We established oral 
      mucosa and skin wound healing models in C57BL/6J mice. RT-PCR, western blotting, 
      immunohistochemistry and immunofluorescence were used to examine the expression 
      of ECM components and key proteins in cell signalling pathways (TGF-beta1, Smad2/3, 
      Cx43, Erk1/2 MMP-1 and collagen III). After injury, buccal mucosa wounds healed 
      with no scar, whereas skin wounds healed with an evident scar. Nevertheless, 
      TGF-beta1 expression gradually increased by the 5th day after injury; Cx43 
      expression showed a similar response, with a progressive increase in the skin and 
      a peak on day 14. In contrast, TGF-beta1 and Cx43 expression in the oral mucosa 
      remained low. The high level of TGF-beta1 increased p-Smad2/3 levels and then 
      induced Cx43, whereas increased expression of Cx43 antagonised the 
      phosphorylation of Erk1/2, a protein downstream of Cx43, which affected MMP-1 
      synthesis. MMP-1 deficiency led to collagen III accumulation and facilitated scar 
      formation. We demonstrated that TGF-beta1-induced Cx43 promotes scar formation via 
      the Erk/MMP-1/collagen III pathway.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Li, Xiaoyan
AU  - Li X
AUID- ORCID: 0000-0001-7166-0135
AD  - Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, 
      Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, China.
FAU - Guo, Lijia
AU  - Guo L
AD  - Department of Orthodontics, School of Stomatology, Capital Medical University, 
      Beijing, China.
FAU - Yang, Xiaohui
AU  - Yang X
AD  - Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, 
      Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, China.
FAU - Wang, Jingyi
AU  - Wang J
AD  - School of Dental Medicine, University of Pennsylvania, Philadelphia, 
      Pennsylvania, USA.
FAU - Hou, Yanan
AU  - Hou Y
AD  - Department of Orthodontics the Third Dental Center, Peking University School of 
      Stomatology, Beijing, China.
FAU - Zhu, Siying
AU  - Zhu S
AD  - Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, 
      Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, China.
FAU - Du, Juan
AU  - Du J
AD  - Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, 
      Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, China.
FAU - Feng, Jie
AU  - Feng J
AD  - Department of General Dentistry, School of Stomatology, Capital Medical 
      University, Beijing, China.
FAU - Xie, Yongmei
AU  - Xie Y
AD  - Department of General Dentistry, School of Stomatology, Capital Medical 
      University, Beijing, China.
FAU - Zhuang, Li
AU  - Zhuang L
AD  - Department of Orthodontics, School of Stomatology, Capital Medical University, 
      Beijing, China.
FAU - He, Xin
AU  - He X
AD  - Department of Orthodontics, School of Stomatology, Capital Medical University, 
      Beijing, China.
FAU - Liu, Yi
AU  - Liu Y
AD  - Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, 
      Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School 
      of Stomatology, Capital Medical University, Beijing, China.
LA  - eng
GR  - 81470751/National Nature Science Foundation of China/
GR  - 81600891/National Nature Science Foundation of China/
GR  - 7172087/Beijing Natural Science Foundation/
GR  - 2016-2-2141/The Capital Health Research and Development of Special/
GR  - ZYLX201703/Beijing Municipal Administration of Hospitals Clinical Medicine 
      Development of Special Funding Support/
GR  - 2017A17/Beijing Baiqianwan Talents Project/
GR  - DFL20181501/Beijing Municipal Administration of Hospitals' Ascent Plan/
GR  - Z16100000516203/Beijing Municipal Science & Technology Commission/
GR  - QML20181501/Beijing Municipal Administration of Hospitals' Youth Programme/
GR  - Beijing Dongcheng Excellent Talent/
PT  - Journal Article
DEP - 20191031
PL  - England
TA  - J Oral Rehabil
JT  - Journal of oral rehabilitation
JID - 0433604
RN  - 0 (Connexin 43)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Cicatrix
MH  - Collagen
MH  - *Connexin 43
MH  - Humans
MH  - Matrix Metalloproteinase 1/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Transforming Growth Factor beta1
OTO - NOTNLM
OT  - Cx43
OT  - TGF-beta1
OT  - collagen III
OT  - scar
EDAT- 2019/06/09 06:00
MHDA- 2020/11/21 06:00
CRDT- 2019/06/09 06:00
PHST- 2019/02/24 00:00 [received]
PHST- 2019/05/07 00:00 [revised]
PHST- 2019/05/26 00:00 [accepted]
PHST- 2019/06/09 06:00 [pubmed]
PHST- 2020/11/21 06:00 [medline]
PHST- 2019/06/09 06:00 [entrez]
AID - 10.1111/joor.12829 [doi]
PST - ppublish
SO  - J Oral Rehabil. 2020 Nov;47 Suppl 1:99-106. doi: 10.1111/joor.12829. Epub 2019 
      Oct 31.