PMID- 31176118 OWN - NLM STAT- MEDLINE DCOM- 20200203 LR - 20200203 IS - 1873-4367 (Electronic) IS - 0927-7765 (Linking) VI - 181 DP - 2019 Sep 1 TI - Poly(amino acid)/ZnO/mesoporous silica nanoparticle based complex drug delivery system with a charge-reversal property for cancer therapy. PG - 461-469 LID - S0927-7765(19)30397-2 [pii] LID - 10.1016/j.colsurfb.2019.05.078 [doi] AB - Negative-to-positive charge-reversal strategy employed in anti-cancer drug delivery systems (DDSs) can improve the utilization of the drugs as well as reduce their side effects efficiently. In this article, a complex DDS named DOX@MSN-ZnO-PLL-PLL(DMA) was prepared. Doxorubicin hydrochloride (DOX) was loaded in mesoporous silica nanoparticles (MSNs), which were then covered by ZnO in situ. Poly-(L)-lysine (PLL) and 2,3-dimethylmaleic anhydride functionalized PLL (PLL(DMA)) were finally coated on the nanoparticles through a Layer-by-Layer (LbL) assembly process with PLL(DMA) outside to obtain the carriers. The negative charged PLL(DMA) avoided the unspecific uptake of the carriers by normal cells at pH 7.4. While the charge-reversal property could reverse the zeta-potential of the carriers to positive in weakly acidic tumor tissues at pH 6.5, which promoted the cytophagy of the carriers by cancer cells. ZnO which blocked the pores of MSNs could be dissolved intracellular due to the more acidic environment in endosome/lysosome, and resulting in drug release for cancer cell apoptosis. Zeta-potential measurements, the in vitro cellular uptake behaviors as well as cellular cytotoxicity of the carriers at different pH values were investigated to prove the charge-reversal property. The in vitro drug release studies and the cellular cytotoxicity studies were also investigated to prove the controlled DOX release behavior of the carriers. In summary, the complex DDS with charge-reversal property should be of consideration in cancer therapy. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Kuang, Ying AU - Kuang Y AD - Glyn O. Philips Hydrocolloid Research Centre at HUT, Hubei University of Technology, Wuhan, Hubei 430068, PR China; Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Chen, Hui AU - Chen H AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Chen, Zhongyin AU - Chen Z AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Wan, Lihui AU - Wan L AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Liu, Jia AU - Liu J AD - Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, PR China. FAU - Xu, Ziqiang AU - Xu Z AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Chen, Xueqin AU - Chen X AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. FAU - Jiang, Bingbing AU - Jiang B AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. Electronic address: bingbingjiang@hubu.edu.cn. FAU - Li, Cao AU - Li C AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, PR China. Electronic address: licao0415@163.com. LA - eng PT - Journal Article DEP - 20190531 PL - Netherlands TA - Colloids Surf B Biointerfaces JT - Colloids and surfaces. B, Biointerfaces JID - 9315133 RN - 0 (Amino Acids) RN - 0 (Antibiotics, Antineoplastic) RN - 0 (Polymers) RN - 7631-86-9 (Silicon Dioxide) RN - 80168379AG (Doxorubicin) RN - SOI2LOH54Z (Zinc Oxide) SB - IM MH - Amino Acids/*chemistry MH - Antibiotics, Antineoplastic/chemistry/*pharmacology MH - Apoptosis/drug effects MH - Cell Proliferation/drug effects MH - Cell Survival/drug effects MH - Doxorubicin/chemistry/*pharmacology MH - *Drug Delivery Systems MH - Drug Liberation MH - Drug Screening Assays, Antitumor MH - HeLa Cells MH - Humans MH - Nanoparticles/*chemistry MH - Particle Size MH - Polymers/*chemistry MH - Porosity MH - Silicon Dioxide/chemistry MH - Surface Properties MH - Zinc Oxide/*chemistry OTO - NOTNLM OT - Cancer therapy OT - Charge-reversal OT - Controlled release OT - Mesoporous silica nanoparticles OT - pH-sensitivity EDAT- 2019/06/09 06:00 MHDA- 2020/02/06 06:00 CRDT- 2019/06/09 06:00 PHST- 2019/01/27 00:00 [received] PHST- 2019/05/06 00:00 [revised] PHST- 2019/05/30 00:00 [accepted] PHST- 2019/06/09 06:00 [pubmed] PHST- 2020/02/06 06:00 [medline] PHST- 2019/06/09 06:00 [entrez] AID - S0927-7765(19)30397-2 [pii] AID - 10.1016/j.colsurfb.2019.05.078 [doi] PST - ppublish SO - Colloids Surf B Biointerfaces. 2019 Sep 1;181:461-469. doi: 10.1016/j.colsurfb.2019.05.078. Epub 2019 May 31.