PMID- 31176251
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20220409
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 181
DP  - 2019 Oct 15
TI  - Small interfering RNA targeting of peroxiredoxinⅡ gene enhances 
      formaldehyde-induced toxicity in bone marrow cells isolated from BALB/c mice.
PG  - 89-95
LID - S0147-6513(19)30627-X [pii]
LID - 10.1016/j.ecoenv.2019.05.086 [doi]
AB  - BACKGROUDS: Formaldehyde (FA) is an important chemicals that can induce sick 
      house syndrome and may be an incentive of childhood leukemia, however the exact 
      mechanism is unclear. Oxidative stress may be an underlying reason of cancer 
      occurring, while diverse antioxidants can protect the bone marrow cells (BMCs) 
      from damaged. PeroxiredoxinⅡ (PrxⅡ) is an important member of the peroxiredoxin 
      family, can remove reactive oxygen species (ROS), and is closely related with the 
      occurrence of tumor. The present study aimed to detect a possible relationship 
      between PrxⅡ gene and FA-induced bone marrow toxicity. METHODS: The BMCs were 
      taken out from BALB/c mice, then exposed to control and different doses of FA 
      (50, 100, 200 mumol/L). The cell viability, ROS level and expressions of PrxⅡ gene 
      were examined. Afterwards, we used a small interfering RNA (siRNA) to inhibit the 
      expression of PrxⅡ gene, and chose 100 mumol/L FA for exposure dose, to examine 
      the cell viability, ROS level, cell cycle, apoptotic rate, expressions of PrxⅡ 
      gene in BMCs. RESULTS: After a 24 h exposure to different doses of FA, the cell 
      viability, expressions of PrxⅡ gene were decreased with the increasing of FA 
      concentration, while the ROS level was increased. Inhibiting PrxⅡ gene's 
      expression could enhance above FA-induced events. Additionally, siRNA targeting 
      of PrxⅡcould aggravate cell cycle arrest to inhibit cell's growth and 
      development, as well as increase apoptotic rates induced by FA. CONCLUSION: These 
      results demonstrated that PrxⅡ gene was involved in FA-induced bone marrow 
      toxicity, and siRNA targeting of PrxⅡcould enhance this toxic process.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Yu, Guangyan
AU  - Yu G
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China. Electronic address: 
      yugy@jlu.edu.cn.
FAU - Zhang, Yixin
AU  - Zhang Y
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
FAU - Liu, Shimeng
AU  - Liu S
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
FAU - Fan, Lida
AU  - Fan L
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
FAU - Yang, Yixue
AU  - Yang Y
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
FAU - Huang, Yulu
AU  - Huang Y
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
FAU - Song, Jiayi
AU  - Song J
AD  - (Department of Occupational and Environmental Health, School of Public Health, 
      Jilin University, Changchun, Jilin Province130021, China.
LA  - eng
PT  - Journal Article
DEP - 20190605
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.11.1.15 (Prdx2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Formaldehyde/*toxicity
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Oxidative Stress
MH  - Peroxiredoxins/antagonists & inhibitors/*genetics/metabolism
MH  - RNA, Small Interfering
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell cycle
OT  - Formaldehyde
OT  - PrxⅡ
OT  - ROS
OT  - siRNA
EDAT- 2019/06/09 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/09 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2019/05/29 00:00 [revised]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/06/09 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2019/06/09 06:00 [entrez]
AID - S0147-6513(19)30627-X [pii]
AID - 10.1016/j.ecoenv.2019.05.086 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Oct 15;181:89-95. doi: 10.1016/j.ecoenv.2019.05.086. 
      Epub 2019 Jun 5.