PMID- 31178726
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 10
DP  - 2019
TI  - Molecular Characterization and Biological Function of a Novel LncRNA CRNG in 
      Swine.
PG  - 539
LID - 10.3389/fphar.2019.00539 [doi]
LID - 539
AB  - Our previous study has showed that a novel gene is differentially expressed in 
      the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, 
      rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that 
      the novel gene is 953 bp without protein-coding ability and locates in chromosome 
      11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named 
      it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) 
      showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in 
      combination with CRNG interference and overexpression showed that the 
      differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, 
      IL-6, and TNF-alpha are mainly involved in viral and pathogens infection and the 
      immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might 
      play a role in preventing the host from being infected by pathogens and viruses 
      and exerting immune regulatory effects in the cytoplasm, which may be involved in 
      prophylaxis of cyadox in piglets.
FAU - Lu, Qirong
AU  - Lu Q
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Li, Li
AU  - Li L
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
FAU - Huang, Aixin
AU  - Huang A
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
FAU - Cui, Luqing
AU  - Cui L
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Zhang, Yinfeng
AU  - Zhang Y
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Liu, Qianying
AU  - Liu Q
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
FAU - Wang, Xu
AU  - Wang X
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Wang, Yulian
AU  - Wang Y
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Liu, Zhenli
AU  - Liu Z
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Yuan, Zonghui
AU  - Yuan Z
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
FAU - Dai, Menghong
AU  - Dai M
AD  - National Reference Laboratory of Veterinary Drug Residues and Ministry of 
      Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in 
      Foods, Huazhong Agricultural University, Wuhan, China.
AD  - Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of 
      Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20190521
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6537671
OTO - NOTNLM
OT  - cyadox
OT  - immune
OT  - inflammation
OT  - lncRNA CRNG
OT  - pathogen infection
EDAT- 2019/06/11 06:00
MHDA- 2019/06/11 06:01
PMCR- 2019/05/21
CRDT- 2019/06/11 06:00
PHST- 2018/11/28 00:00 [received]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/06/11 06:00 [entrez]
PHST- 2019/06/11 06:00 [pubmed]
PHST- 2019/06/11 06:01 [medline]
PHST- 2019/05/21 00:00 [pmc-release]
AID - 10.3389/fphar.2019.00539 [doi]
PST - epublish
SO  - Front Pharmacol. 2019 May 21;10:539. doi: 10.3389/fphar.2019.00539. eCollection 
      2019.