PMID- 31178841
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 10
DP  - 2019
TI  - Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, 
      Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate.
PG  - 1128
LID - 10.3389/fmicb.2019.01128 [doi]
LID - 1128
AB  - Viral entry mechanisms of herpesviruses constitute a highly complex process which 
      implicates several viral glycoproteins and different receptors on the host cell 
      surfaces. This initial infection stage was currently undescribed for Ostreid 
      herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific 
      oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the 
      attachment of the virus to oyster cells, three viral putative membrane proteins, 
      encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against 
      these targets were used to explore first interactions between the virus and host 
      cells. In addition, effects of dextran sulfate, a negative charged sulfated 
      polysaccharide, were investigated on OsHV-1 infection. Effects of antiviral 
      antibodies and dextran sulfate were evaluated by combining viral DNA and RNA 
      detection in spat (in vivo trials) and in oyster hemolymph (in vitro trials). 
      Results showed that viral protein encoded by ORF 25 appeared to be involved in 
      interaction between OsHV-1 and host cells even if other proteins are likely 
      implicated, such as proteins encoded by ORF 72 and ORF 41. Dextran sulfate at 30 
      mug/mL significantly reduced the spat mortality rate in the experimental 
      conditions. Taken together, these results contribute to better understanding the 
      pathogenesis of the viral infection, especially during the first stage of OsHV-1 
      infection, and open the way toward new approaches to control OsHV-1 infection in 
      confined facilities.
FAU - Martenot, Claire
AU  - Martenot C
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, La Tremblade, France.
FAU - Faury, Nicole
AU  - Faury N
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, La Tremblade, France.
FAU - Morga, Benjamin
AU  - Morga B
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, La Tremblade, France.
FAU - Degremont, Lionel
AU  - Degremont L
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, La Tremblade, France.
FAU - Lamy, Jean-Baptiste
AU  - Lamy JB
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, La Tremblade, France.
FAU - Houssin, Maryline
AU  - Houssin M
AD  - LABEO, Saint Contest, France.
FAU - Renault, Tristan
AU  - Renault T
AD  - Departement Ressources Biologiques et Environnement, Institut Francais de 
      Recherche pour l'Exploitation de la Mer, Nantes, France.
LA  - eng
PT  - Journal Article
DEP - 20190524
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6543491
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - OsHV-1
OT  - antiviral antibodies
OT  - dextran sulfate
OT  - infection
OT  - interaction
EDAT- 2019/06/11 06:00
MHDA- 2019/06/11 06:01
PMCR- 2019/05/24
CRDT- 2019/06/11 06:00
PHST- 2019/02/21 00:00 [received]
PHST- 2019/05/03 00:00 [accepted]
PHST- 2019/06/11 06:00 [entrez]
PHST- 2019/06/11 06:00 [pubmed]
PHST- 2019/06/11 06:01 [medline]
PHST- 2019/05/24 00:00 [pmc-release]
AID - 10.3389/fmicb.2019.01128 [doi]
PST - epublish
SO  - Front Microbiol. 2019 May 24;10:1128. doi: 10.3389/fmicb.2019.01128. eCollection 
      2019.