PMID- 31179576
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20191111
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 48
IP  - 2
DP  - 2019 Jun
TI  - Evaluation of automated erythrocyte methodology in new world camelids using the 
      ADVIA 2120 hematology analyzer.
PG  - 239-249
LID - 10.1111/vcp.12733 [doi]
AB  - BACKGROUND: Accurate erythrocyte measurements with ADVIA hematology analyzers 
      require isovolumetric cell sphering in one reaction and hemolysis in another. 
      However, camelid erythrocytes are resistant to sphering and osmotic lysis, and no 
      published evaluation of ADVIA methods for camelids exists. OBJECTIVES: The 
      objectives were to demonstrate whether camelid erythrocytes sphere in the ADVIA 
      red blood cell/platelet (RBC/PLT) reagent and lyse in the ADVIA cyanide HGB 
      reagent, and to determine optimal ADVIA settings for camelids. METHODS: Camelid 
      and canine blood were diluted to 1:625 in RBC/PLT reagent and evaluated 
      microscopically for erythrocyte sphering. A camelid sample was incubated with the 
      hemoglobin (HGB) reagent at varying dilutions to evaluate hemolysis. The RBC, 
      hematocrit (HCT), mean cell volume (MCV), and mean corpuscular hemoglobin 
      concentration (MCHC) using three ADVIA species settings (equine, bovine, and 
      caprine) were compared to their respective reference methods: Z2 Coulter 
      impedance counter, packed cell volume, calculated MCV (PCV x 10/Coulter RBC), and 
      calculated MCHC (HGB x 100/PCV). Reference MCV was also compared to MCV 
      calculated using the ADVIA equine RBC count. Comparisons were assessed using 
      Passing-Bablok regression and Bland-Altman difference plots. RESULTS: Camelid 
      erythrocytes did not sphere in the RBC/PLT reagent, but did lyse in the HGB 
      reagent. The ADVIA equine setting RBC count was acceptably close to the Coulter 
      count. Hematocrit, MCV, and MCHC from all settings were significantly different 
      from the reference methods. Mean cell volumes calculated using the equine setting 
      RBC counts were acceptably close to the reference MCV. CONCLUSIONS: Camelid ADVIA 
      erythrogram results should be reported as follows: RBC counts and HGB 
      concentrations using the equine setting, spun PCVs, MCVs calculated using the PCV 
      and equine setting RBC, and MCHCs calculated using the PCV and equine setting 
      HGB.
CI  - (c) 2019 American Society for Veterinary Clinical Pathology.
FAU - Viesselmann, Lisa C
AU  - Viesselmann LC
AD  - Department of Biomedical and Diagnostic Sciences, University of Tennessee, 
      Knoxville, TN.
FAU - Flatland, Bente
AU  - Flatland B
AUID- ORCID: 0000-0003-0956-5895
AD  - Department of Biomedical and Diagnostic Sciences, University of Tennessee, 
      Knoxville, TN.
FAU - Stokol, Tracy
AU  - Stokol T
AD  - Department of Population Medicine and Diagnostic Sciences, Cornell University, 
      Ithaca, NY.
FAU - Sisson, Sandra
AU  - Sisson S
AD  - Department of Population Medicine and Diagnostic Sciences, Cornell University, 
      Ithaca, NY.
FAU - Schaefer, Deanna M W
AU  - Schaefer DMW
AUID- ORCID: 0000-0002-5260-2180
AD  - Department of Biomedical and Diagnostic Sciences, University of Tennessee, 
      Knoxville, TN.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20190609
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
SB  - IM
MH  - Animals
MH  - Camelids, New World/*blood
MH  - Dogs/blood
MH  - Erythrocytes/cytology
MH  - Hematocrit/veterinary
MH  - Hematologic Tests/*instrumentation
MH  - Horses/blood
MH  - Reference Values
OTO - NOTNLM
OT  - allowable total error
OT  - alpaca
OT  - bias
OT  - llama
OT  - red blood cells
EDAT- 2019/06/11 06:00
MHDA- 2019/11/12 06:00
CRDT- 2019/06/11 06:00
PHST- 2018/09/12 00:00 [received]
PHST- 2018/11/13 00:00 [revised]
PHST- 2018/11/15 00:00 [accepted]
PHST- 2019/06/11 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2019/06/11 06:00 [entrez]
AID - 10.1111/vcp.12733 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2019 Jun;48(2):239-249. doi: 10.1111/vcp.12733. Epub 2019 Jun 9.