PMID- 31181347
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20191217
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 125
DP  - 2019 Aug
TI  - Evaluation of three commercial porcine reproductive and respiratory syndrome 
      virus (PRRSV) oral fluid antibody ELISAs using samples of known status.
PG  - 113-118
LID - S0034-5288(19)30347-9 [pii]
LID - 10.1016/j.rvsc.2019.05.019 [doi]
AB  - Oral fluid (n = 564) samples collected longitudinally from twelve 14-week-old 
      pigs vaccinated with a porcine reproductive and respiratory syndrome virus 
      (PRRSV) modified live vaccine were used to evaluate and compare the diagnostic 
      performance of three commercial PRRSV oral fluid (OF) ELISAs (ELISAs 1, 2, 3). 
      Serum samples (n = 132) tested by a PRRSV serum ELISA (ELISA 'S') provided an 
      antibody response baseline for comparison. The initial analysis comparing the 
      rate of positivity between each OF ELISA versus ELISA 'S' and then pairwise among 
      the three OF ELISAs determined that ELISA 2 (143 false negative results) was 
      significantly different from ELISAs 1 and 3, and from ELISA 'S' (Cochran's Q 
      test, p < 0.05). Receiver operating characteristic (ROC) analyses based on the 
      manufacturers' recommended cutoff were used to estimate the diagnostic 
      sensitivities and specificities of ELISA 1 (100%, 100%), ELISA 2 (62%, 97%), and 
      ELISA 3 (94%, 100%). As an additional aid for interpreting results, the 
      diagnostic sensitivities and specificities of each OF ELISA were also estimated 
      over a range of cutoffs. Area under the curve comparisons found no significant 
      difference between ELISAs 1 and 3, but ELISA 2 differed from both ELISA 1 and 3 
      (ROC Chi-square, p < 0.05). Based on these analyses, the overall diagnostic 
      performance of the three OF ELISAs ranked ELISA 1 >/= ELISA 3 > ELISA 2.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Henao-Diaz, Alexandra
AU  - Henao-Diaz A
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. Electronic 
      address: yulyh@iastate.edu.
FAU - Gimenez-Lirola, Luis
AU  - Gimenez-Lirola L
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Magtoto, Ronaldo
AU  - Magtoto R
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Ji, Ju
AU  - Ji J
AD  - Department of Statistics, College of Liberal Arts and Sciences, Iowa State 
      University, Ames, IA 50011, USA.
FAU - Zimmerman, Jeffrey
AU  - Zimmerman J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20190603
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - ROC Curve
MH  - Saliva/*virology
MH  - Sensitivity and Specificity
MH  - Serum/virology
MH  - Swine
OTO - NOTNLM
OT  - Diagnostic sensitivity
OT  - Diagnostic specificity
OT  - ELISA
OT  - Oral fluids
OT  - PRRSV
EDAT- 2019/06/11 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/06/11 06:00
PHST- 2019/04/23 00:00 [received]
PHST- 2019/05/29 00:00 [revised]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/06/11 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/06/11 06:00 [entrez]
AID - S0034-5288(19)30347-9 [pii]
AID - 10.1016/j.rvsc.2019.05.019 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Aug;125:113-118. doi: 10.1016/j.rvsc.2019.05.019. Epub 2019 Jun 
      3.