PMID- 31186161
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20211204
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 46
IP  - 5
DP  - 2019 May 20
TI  - mTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through 
      augmented GSK-3beta/MMPs.
PG  - 235-245
LID - S1673-8527(19)30085-2 [pii]
LID - 10.1016/j.jgg.2019.03.013 [doi]
AB  - Golgi membrane protein 1 (GOLM1/GP73) is a serum marker of hepatocellular 
      carcinoma (HCC). We have previously shown that mTOR promoted tumorigenesis of HCC 
      through stimulating GOLM1 expression. In this study, we demonstrated that the 
      mammalian target of rapamycin (mTOR) was a negative regulator of microRNA-145 
      (miR-145) expression. miR-145 inhibited GOLM1 expression by targeting a coding 
      sequence of GOLM1 gene. GOLM1 and miR-145 were inversely correlated in human HCC 
      tissues. GOLM1-enriched exosomes activated the glycogen synthase kinase-3beta/matrix 
      metalloproteinases (GSK-3beta/MMPs) signaling axis of recipient cells and 
      accelerated cell proliferation and migration. In contrast, miR-145 suppressed 
      tumorigenesis and metastasis. We suggest that mTOR/miR-145/GOLM1 signaling 
      pathway should be targeted for HCC treatment.
CI  - Copyright (c) 2019 Institute of Genetics and Developmental Biology, Chinese Academy 
      of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Gai, Xiaochen
AU  - Gai X
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Tang, Bufu
AU  - Tang B
AD  - First Affiliated Hospital, Dalian Medical University, Dalian, 116011, China; 
      Department of Radiology, Lishui Hospital of Zhejiang University, Lishui, 323000, 
      China; Department of Radiology, Second Affiliated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, 323000, China.
FAU - Liu, Fangming
AU  - Liu F
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Wu, Yuting
AU  - Wu Y
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Wang, Fang
AU  - Wang F
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Jing, Yanling
AU  - Jing Y
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Huang, Fuqiang
AU  - Huang F
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China.
FAU - Jin, Di
AU  - Jin D
AD  - Institute of Cancer Stem Cell, Dalian Medical University, Dalian, 116044, China.
FAU - Wang, Ling
AU  - Wang L
AD  - First Affiliated Hospital, Dalian Medical University, Dalian, 116011, China. 
      Electronic address: lingwang2006@hotmail.com.
FAU - Zhang, Hongbing
AU  - Zhang H
AD  - State Key Laboratory of Medical Molecular Biology, Department of Physiology, 
      Institute of Basic Medical Sciences and School of Basic Medicine, Graduate School 
      of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 
      100005, China; Department of Neurology, Institute of Neural Regeneration and 
      Repair, The First People's Hospital of Yichang, College of Medicine, Three Gorges 
      University, Yichang, 443000, China. Electronic address: hbzhang@ibms.pumc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190518
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
RN  - 0 (GOLM1 protein, human)
RN  - 0 (MIRN145 microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Carcinogenesis/genetics
MH  - Carcinoma, Hepatocellular/*pathology
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Exosomes/metabolism
MH  - Glycogen Synthase Kinase 3 beta/*metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/*pathology
MH  - Matrix Metalloproteinases/*metabolism
MH  - Membrane Proteins/*metabolism
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis/genetics
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Exosome
OT  - GOLM1
OT  - Hepatocellular carcinoma
OT  - mTOR
OT  - miR-145
EDAT- 2019/06/13 06:00
MHDA- 2020/06/23 06:00
CRDT- 2019/06/13 06:00
PHST- 2018/10/12 00:00 [received]
PHST- 2019/03/06 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/06/13 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/06/13 06:00 [entrez]
AID - S1673-8527(19)30085-2 [pii]
AID - 10.1016/j.jgg.2019.03.013 [doi]
PST - ppublish
SO  - J Genet Genomics. 2019 May 20;46(5):235-245. doi: 10.1016/j.jgg.2019.03.013. Epub 
      2019 May 18.