PMID- 31186702
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 17
IP  - 6
DP  - 2019 Jun
TI  - Long non-coding RNA NEF inhibits proliferation and promotes apoptosis of 
      laryngeal squamous cell carcinoma cells by inhibiting Wnt/beta-catenin signaling.
PG  - 4928-4934
LID - 10.3892/ol.2019.10150 [doi]
AB  - The present study aimed to investigate the involvement of the recently identified 
      long non-coding RNA neighboring enhancer of FOXA2 (lncRNA NEF) in laryngeal 
      squamous cell carcinoma (LSCC). In this study, the expression levels of lncRNA 
      NEF in tumor tissues and paired adjacent normal tissues were detected by reverse 
      transcription-quantitative polymerase chain reaction (RT-qPCR). Serum levels of 
      NEF in patients with LSCC and healthy controls were also measured using RT-qPCR. 
      Clinical and follow-up data of patients with LSCC were retrospectively analyzed. 
      Diagnostic and prognostic values of serum NEF were evaluated by receiver 
      operating characteristic curve and survival curve analysis, respectively. In 
      addition, a NEF expression vector was constructed and transfected into human LSCC 
      cells. The effects of NEF overexpression on cell proliferation, apoptosis and 
      beta-catenin expression were explored by Cell Counting kit-8 cell proliferation 
      assay, MTT assay and western blotting. NEF was significantly downregulated in 
      tumor tissues compared with in paired adjacent normal tissues of patients with 
      LSCC. Serum levels of NEF were significantly lower in patients with LSCC than in 
      healthy controls. Low serum levels of NEF distinguished patients with LSCC from 
      healthy controls, and also indicated shorter postoperative survival. NEF 
      overexpression inhibited proliferation and promoted apoptosis of LSCC cells, and 
      also downregulated beta-catenin expression. No significant effects of Wnt agonist on 
      NEF expression were identified; however, Wnt agonist reduced the effects of NEF 
      overexpression on cancer cell proliferation and apoptosis. In conclusion, lncRNA 
      NEF may inhibit proliferation and promote apoptosis of LSCC cells by inhibiting 
      Wnt/beta-catenin signaling.
FAU - Cui, Xiangyan
AU  - Cui X
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University, Changchun, Jilin 130021, P.R. China.
FAU - Fang, Ning
AU  - Fang N
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University, Changchun, Jilin 130021, P.R. China.
FAU - Cui, Yu
AU  - Cui Y
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University, Changchun, Jilin 130021, P.R. China.
FAU - Xiao, Dong
AU  - Xiao D
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University, Changchun, Jilin 130021, P.R. China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin 
      University, Changchun, Jilin 130021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190315
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6507471
OTO - NOTNLM
OT  - Wnt/beta-catenin signaling
OT  - apoptosis
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding RNA neighboring enhancer of FOXA2
OT  - proliferation
EDAT- 2019/06/13 06:00
MHDA- 2019/06/13 06:01
PMCR- 2019/03/15
CRDT- 2019/06/13 06:00
PHST- 2018/06/27 00:00 [received]
PHST- 2019/01/25 00:00 [accepted]
PHST- 2019/06/13 06:00 [entrez]
PHST- 2019/06/13 06:00 [pubmed]
PHST- 2019/06/13 06:01 [medline]
PHST- 2019/03/15 00:00 [pmc-release]
AID - OL-0-0-10150 [pii]
AID - 10.3892/ol.2019.10150 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 Jun;17(6):4928-4934. doi: 10.3892/ol.2019.10150. Epub 2019 Mar 
      15.