PMID- 31189122
OWN - NLM
STAT- MEDLINE
DCOM- 20200106
LR  - 20200106
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 135
DP  - 2019 Sep 1
TI  - Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine 
      oocyte.
PG  - 19-24
LID - S0093-691X(19)30135-9 [pii]
LID - 10.1016/j.theriogenology.2019.05.011 [doi]
AB  - Approximately 40% of mammalian genome is made of transposable elements (TEs), and 
      during specific biological processes, such as gametogenesis, they may be 
      activated by global demethylation, so strict silencing mechanism is indispensable 
      for genomic stability. Here, we performed small RNA-seq on Dicer1 knockdown (KD) 
      oocytes in pig, and observed short interspersed nuclear elements 1B (SINE1B) 
      derived endogenous small interfering RNAs (endo-siRNAs), termed SINE1B-siRNAs, 
      were significantly decreased and their biogenesis was dependent on Dicer1 and 
      transcript of SINE1B. Furthermore, by injection of mimics and inhibitors of the 
      SINE1B-siRNAs into germinal vesicle-stage (GV-stage) oocytes, we found the 
      maturation rate was significantly decreased by SINE1B-siRNAs, indicating the 
      SINE1B-siRNAs are indispensible for in vitro maturation (IVM) of porcine oocyte. 
      To figure out the mechanism, we checked the expression pattern and DNA 
      methylation status of SINE1B during IVM of porcine oocytes, and demonstrated the 
      SINE1B-siRNAs could repress SINE1B expression induced by hypomethylation at a 
      post-transcriptional level. Our results suggest that during gametogenesis when 
      the erasure of DNA methylation occurs, endo-siRNAs act as a chronic response to 
      limit retrotransposon activation.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Kong, Qing-Ran
AU  - Kong QR
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China. Electronic address: kqr086@neau.edu.cn.
FAU - Zhang, Jia-Ming
AU  - Zhang JM
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Zhang, Xiao-Lei
AU  - Zhang XL
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Zong, Ming
AU  - Zong M
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Zheng, Kai-Lun
AU  - Zheng KL
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Liu, Lei
AU  - Liu L
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Meng, Fan-Xi
AU  - Meng FX
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Zhang, Ting-Ting
AU  - Zhang TT
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Weng, Xiao-Gang
AU  - Weng XG
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China.
FAU - Mu, Yan-Shuang
AU  - Mu YS
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China. Electronic address: muyanshuang@163.com.
FAU - Liu, Zhong-Hua
AU  - Liu ZH
AD  - Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang 
      Province, College of Life Science, Northeast Agricultural University, Harbin, 
      150030, Heilongjiang, PR China. Electronic address: liuzhonghua@neau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190522
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Retroelements)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - DEAD-box RNA Helicases/genetics/metabolism
MH  - DNA Methylation
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - In Vitro Oocyte Maturation Techniques/*veterinary
MH  - Oocytes/*physiology
MH  - RNA, Small Interfering
MH  - Retroelements
MH  - Short Interspersed Nucleotide Elements/genetics/*physiology
MH  - Swine
OTO - NOTNLM
OT  - IVM
OT  - Oocyte
OT  - Pig
OT  - SINE1B
OT  - endo-siRNAs
EDAT- 2019/06/13 06:00
MHDA- 2020/01/07 06:00
CRDT- 2019/06/13 06:00
PHST- 2018/12/07 00:00 [received]
PHST- 2019/04/20 00:00 [revised]
PHST- 2019/05/13 00:00 [accepted]
PHST- 2019/06/13 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
PHST- 2019/06/13 06:00 [entrez]
AID - S0093-691X(19)30135-9 [pii]
AID - 10.1016/j.theriogenology.2019.05.011 [doi]
PST - ppublish
SO  - Theriogenology. 2019 Sep 1;135:19-24. doi: 10.1016/j.theriogenology.2019.05.011. 
      Epub 2019 May 22.