PMID- 31189757
OWN - NLM
STAT- MEDLINE
DCOM- 20200211
LR  - 20200225
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 81
IP  - 8
DP  - 2019 Aug 24
TI  - Determination of antibody induction by highly pathogenic porcine reproductive and 
      respiratory syndrome virus (HP-PRRSV) vaccine: A comparison of two ELISA kits.
PG  - 1173-1176
LID - 10.1292/jvms.18-0482 [doi]
AB  - Two commercial porcine reproductive and respiratory syndrome virus (PRRSV) 
      antibody ELISA kits (IDEXX and LSI) are currently in extensive use. To determine 
      which kit is more suitable for the evaluation of HP-PRRSV vaccine efficacy, the 
      two kits were used to test 546 serum samples. The agreement between the results 
      was unsatisfactory, with a kappa statistic of 0.681 and a linear correlation 
      coefficient of 0.665. In tests of samples from experimentally vaccinated and 
      PRRSV-negative herds, IDEXX-ELISA identified antibody-positive conversion earlier 
      and showed a higher specificity compared to LSI-ELISA. The serological profile 
      obtained by neutralization testing was closer to that obtained by IDEXX-ELISA 
      than by LSI-ELISA in the late immunization period. The findings reveal that 
      IDEXX-ELISA is the more suitable for the evaluation of antibody response to 
      HP-PRRSV vaccine and for guiding vaccination strategies.
FAU - Ge, Meng
AU  - Ge M
AD  - Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 
      Changchun 130122, China.
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, 
      China.
FAU - Li, Run-Cheng
AU  - Li RC
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, 
      China.
FAU - Gong, Wenjie
AU  - Gong W
AD  - Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 
      Changchun 130122, China.
FAU - Tu, Changchun
AU  - Tu C
AD  - Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 
      Changchun 130122, China.
LA  - eng
PT  - Journal Article
DEP - 20190613
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - *Antibodies, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Neutralization Tests/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology/prevention & 
      control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccination/veterinary
MH  - Viral Vaccines/*immunology
PMC - PMC6715930
OTO - NOTNLM
OT  - ELISA
OT  - antibody
OT  - evaluation
OT  - highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) 
      vaccine
EDAT- 2019/06/14 06:00
MHDA- 2020/02/12 06:00
PMCR- 2019/08/01
CRDT- 2019/06/14 06:00
PHST- 2019/06/14 06:00 [pubmed]
PHST- 2020/02/12 06:00 [medline]
PHST- 2019/06/14 06:00 [entrez]
PHST- 2019/08/01 00:00 [pmc-release]
AID - 18-0482 [pii]
AID - 10.1292/jvms.18-0482 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2019 Aug 24;81(8):1173-1176. doi: 10.1292/jvms.18-0482. Epub 2019 
      Jun 13.