PMID- 31190174
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 300
IP  - 2
DP  - 2019 Aug
TI  - Therapeutic effect of human umbilical cord mesenchymal stem cells on tubal factor 
      infertility using a chronic salpingitis murine model.
PG  - 421-429
LID - 10.1007/s00404-019-05209-6 [doi]
AB  - BACKGROUND: The study was conducted to evaluate the application of human 
      umbilical cord mesenchymal stem cells (hUCMSCs) in the treatment of tubal factor 
      infertility (TFI) caused by Chlamydia trachomatis, and investigate their effect 
      on fertility in animal models of chronic salpingitis. METHODS: In this study, we 
      investigated the therapy effects of the transplantation of hUCMSCs in tubal 
      factor infertility using a chronic salpingitis murine model which induced 
      Chlamydia trachomatis. Twenty rats were divided into two groups: control group 
      (n = 10) and treatment group (n = 10). hUCMSCs were given to mice after exposure 
      to C. trachomatis for 4 weeks. After treatment for 4 weeks, five mice were 
      randomly selected from each of the two groups to sacrifice and we examined the 
      organ morphology and pathology, inflammatory cytokines, proliferation, and 
      apoptosis in fallopian tube (FT).The remaining five mice from each group were 
      caged 2:1 with male mice for another 4 weeks, the numbers of pregnant mice and 
      the mean number of pups in the different groups were enumerated and calculated. 
      RESULTS: Intravaginal inoculation of hUCMSCs alleviated hydrosalpinx of the 
      oviduct. EdU-labeled hUCMSCs are located at the interstitial site of the 
      fallopian tube. Macrophage (F4/80) infiltration was significantly reduced in the 
      treatment group compared with the control group and expression levels of the 
      anti-inflammatory cytokine IL10 were increased after hUCMSCs treatment. 
      Furthermore, mRNA and protein expression levels of PCNA and Caspase-3 were 
      increased and decreased, respectively, in the hUCMSCs' treatment group compared 
      with the control. Moreover, hUCMSCs' transplantation improved murine fertility. 
      CONCLUSIONS: Anti-inflammatory and anti-apoptotic effects of hUCMSCs may play an 
      important role in TFI. Our data suggest that hUCMSCs' transplantation contributed 
      to the repair of tubal injury and improvement of fertility, providing a basis for 
      assessing the contribution of stem cells in the oviduct for direct repair of the 
      tube to assist reproduction.
FAU - Liao, Wenjuan
AU  - Liao W
AD  - Department of Obstetrics and Gynecology, Third Affiliated Hospital of Sun Yat-Sen 
      University, 600 Tianhe Road, Tianhe District, Guangzhou, 510630, Guangdong, 
      China.
FAU - Tang, Xinran
AU  - Tang X
AD  - Department of Obstetrics and Gynecology, Third Affiliated Hospital of Sun Yat-Sen 
      University, 600 Tianhe Road, Tianhe District, Guangzhou, 510630, Guangdong, 
      China.
FAU - Li, Xiaomao
AU  - Li X
AUID- ORCID: 0000-0001-6374-1488
AD  - Department of Obstetrics and Gynecology, Third Affiliated Hospital of Sun Yat-Sen 
      University, 600 Tianhe Road, Tianhe District, Guangzhou, 510630, Guangdong, 
      China. lixmao@mail.sysu.edu.cn.
FAU - Li, Tian
AU  - Li T
AD  - Department of Obstetrics and Gynecology, Third Affiliated Hospital of Sun Yat-Sen 
      University, 600 Tianhe Road, Tianhe District, Guangzhou, 510630, Guangdong, 
      China.
LA  - eng
GR  - 320.6755.15012/Wu Jieping Fund Medical Association/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190612
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
SB  - IM
MH  - Animals
MH  - Chlamydia trachomatis/*pathogenicity
MH  - Cord Blood Stem Cell Transplantation/*methods
MH  - Disease Models, Animal
MH  - Fallopian Tubes/transplantation
MH  - Female
MH  - Humans
MH  - Infertility/*therapy
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Rats
MH  - Salpingitis/*complications
OTO - NOTNLM
OT  - Anti-apoptotic
OT  - Anti-inflammatory
OT  - Human umbilical cord mesenchymal stem cells
OT  - Tubal factor infertility
EDAT- 2019/06/14 06:00
MHDA- 2020/04/28 06:00
CRDT- 2019/06/14 06:00
PHST- 2018/11/14 00:00 [received]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/06/14 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PHST- 2019/06/14 06:00 [entrez]
AID - 10.1007/s00404-019-05209-6 [pii]
AID - 10.1007/s00404-019-05209-6 [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2019 Aug;300(2):421-429. doi: 10.1007/s00404-019-05209-6. 
      Epub 2019 Jun 12.