PMID- 31190648
OWN - NLM
STAT- MEDLINE
DCOM- 20200124
LR  - 20200124
IS  - 1475-2670 (Electronic)
IS  - 0007-4853 (Linking)
VI  - 110
IP  - 1
DP  - 2020 Feb
TI  - Habitat visualization, acquisition features and necessity of the 
      gammaproteobacterial symbiont of pistachio stink Bug, Acrosternum heegeri (Hem.: 
      Pentatomidae).
PG  - 22-33
LID - 10.1017/S0007485319000245 [doi]
AB  - Plant-sucking stinkbugs are especially associated with mutualistic gut bacterial 
      symbionts. Here, we explored the symbiotic relationship of a pistachio stinkbug, 
      Acrosternum heegeri Fieber by histological, fluorescence in situ hybridization 
      (FISH), real-time PCR and molecular phylogenetic techniques. Furthermore, the 
      effects of the symbiont on the resting/wandering behaviors of the newborn nymphs, 
      pre-adult survival rates, and stage compositions were investigated. Transmission 
      electron microscopy and real-time PCR analyses showed that a rod-shaped 
      gammaproteobacterium was persistently located within the posterior midgut crypts. 
      Molecular phylogenetic and FISH techniques strongly suggested that this symbiont 
      should be placed in the genus Pantoea of the Enterobacteriales. Scanning electron 
      microscopy confirmed the presence of the bacterial cells on the egg surface which 
      the surface sterilization of the eggs resulted in the successful removal of the 
      symbiont from the eggs. Symbiotic and aposymbiotic A. heegeri showed no 
      significant differences in the wandering behaviors of the first nymphal stages, 
      while the symbiont-free insects suffered retarded growth and lower survivability. 
      Together, the results highlight the habitat and acquisition features of Pantoea 
      symbiont and its contribution in A. heegeri biology that might help us for better 
      pest management in the future.
FAU - Kashkouli, M
AU  - Kashkouli M
AD  - Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, 
      P.O.Box 14115-336, Tehran, Iran.
FAU - Fathipour, Y
AU  - Fathipour Y
AUID- ORCID: 0000-0002-7963-5409
AD  - Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, 
      P.O.Box 14115-336, Tehran, Iran.
FAU - Mehrabadi, M
AU  - Mehrabadi M
AD  - Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, 
      P.O.Box 14115-336, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20190613
PL  - England
TA  - Bull Entomol Res
JT  - Bulletin of entomological research
JID - 2984715R
SB  - IM
MH  - Animals
MH  - Female
MH  - Gammaproteobacteria/*isolation & purification
MH  - Gastrointestinal Tract/microbiology/ultrastructure
MH  - Heteroptera/*microbiology/ultrastructure
MH  - Ovary/microbiology
MH  - Ovum/microbiology
MH  - Symbiosis
OTO - NOTNLM
OT  - Heritable symbiont
OT  - gammaproteobacteria
OT  - pentatomidae
OT  - symbiont elimination
EDAT- 2019/06/14 06:00
MHDA- 2020/01/25 06:00
CRDT- 2019/06/14 06:00
PHST- 2019/06/14 06:00 [pubmed]
PHST- 2020/01/25 06:00 [medline]
PHST- 2019/06/14 06:00 [entrez]
AID - S0007485319000245 [pii]
AID - 10.1017/S0007485319000245 [doi]
PST - ppublish
SO  - Bull Entomol Res. 2020 Feb;110(1):22-33. doi: 10.1017/S0007485319000245. Epub 
      2019 Jun 13.