PMID- 31193181 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 2215-0161 (Print) IS - 2215-0161 (Electronic) IS - 2215-0161 (Linking) VI - 6 DP - 2019 TI - Laboratory method of microbial induced solidification/stabilization for municipal solid waste incineration fly ash. PG - 1036-1043 LID - 10.1016/j.mex.2019.05.006 [doi] AB - This paper presents a method to solidify/stabilize the municipal solid waste incineration (MSWI) fly ash by originally employing the microbial induced carbonate precipitation (MICP) technique. In this method, the rich endogenous calcium in the MSWI fly ash was utilized to induce calcite precipitation, which is different from the operation of adding extra calcium source in previous researches. The fly ash sample had a CaO content of 44.5%, and its leaching concentrations of Zn, Cr and Pb exceed the limits of the identification standard for hazardous wastes in China. The optical density at 600 nm (OD600) of the bacterial solution was about 1.0 after the processes of bacterial activation and reproduction. The prepared fly ash sample was well mixed with bacterial solution at an ash-liquid ratio of 1 kg: 0.3 L and cured at a temperature of 20 degrees C and a humidity of >/=95% for 7 days. After treatment, the heavy metal leachability significantly reduced to meet the standard for pollution control of landfill site, and the unconfined compressive strength increased approximately 40%. The precipitated carbonates were verified by SEM-EDS analysis and quantified by measurement of carbonate content via acid-dissolving method. The results shone a light on the possibility of using MICP technique as a useful and efficient tool to stabilize the MSWI fly ash before being reused or properly stored in landfills. *The MICP method is efficient to reduce the heavy metal leachability and increase the compressive strength of MSWI fly ash.*The endogenous calcium in MSWI fly ash was utilized to induce calcite precipitation.*The heavy metals in MSWI fly ash were well immobilized by the formation of carbonates. FAU - Xu, Hui AU - Xu H AD - School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China. FAU - Zheng, Hao AU - Zheng H AD - School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China. FAU - Wang, Jin-Nan AU - Wang JN AD - School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China. FAU - Ding, Xiao-Qing AU - Ding XQ AD - School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China. FAU - Chen, Ping AU - Chen P AD - School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China. LA - eng PT - Journal Article DEP - 20190507 PL - Netherlands TA - MethodsX JT - MethodsX JID - 101639829 PMC - PMC6520556 OTO - NOTNLM OT - Compressive strength OT - Endogenous calcium OT - Heavy metal leachability OT - Microbial induced carbonate precipitation (MICP) OT - Microbial induced solidification/stabilization method OT - Municipal solid waste incineration (MSWI) fly ash OT - Solidification/stabilization method EDAT- 2019/06/14 06:00 MHDA- 2019/06/14 06:01 PMCR- 2019/05/07 CRDT- 2019/06/14 06:00 PHST- 2018/09/27 00:00 [received] PHST- 2019/05/04 00:00 [accepted] PHST- 2019/06/14 06:00 [entrez] PHST- 2019/06/14 06:00 [pubmed] PHST- 2019/06/14 06:01 [medline] PHST- 2019/05/07 00:00 [pmc-release] AID - S2215-0161(19)30125-6 [pii] AID - 10.1016/j.mex.2019.05.006 [doi] PST - epublish SO - MethodsX. 2019 May 7;6:1036-1043. doi: 10.1016/j.mex.2019.05.006. eCollection 2019.