PMID- 31195760
OWN - NLM
STAT- MEDLINE
DCOM- 20191129
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 11
DP  - 2019 Jun 5
TI  - Hispidulin Inhibits Mast Cell-Mediated Allergic Inflammation through 
      Down-Regulation of Histamine Release and Inflammatory Cytokines.
LID - 10.3390/molecules24112131 [doi]
LID - 2131
AB  - Hispidulin (4',5,7-trihydroxy-6-methoxyflavone) is a natural compound derived 
      from traditional Chinese medicinal herbs, and it is known to have an 
      anti-inflammatory effect. Here, we investigated the effect of hispidulin on the 
      immunoglobulin E (IgE)-mediated allergic responses in rat basophilic leukemia 
      (RBL)-2H3 mast cells. When RBL-2H3 cells were sensitized with anti-dinitrophenyl 
      (anti-DNP) IgE and subsequently stimulated with DNP-human serum albumin (HSA), 
      histamine and beta-hexosaminidase were released from the cells by degranulation of 
      activated mast cells. However, pretreatment with hispidulin before the 
      stimulation of DNP-HSA markedly attenuated release of both in anti-DNP 
      IgE-sensitized cells. Furthermore, we investigated whether hispidulin inhibits 
      anti-DNP IgE and DNP-HSA-induced passive cutaneous anaphylaxis (PCA), as an 
      animal model for Type I allergies. Hispidulin markedly decreased the PCA reaction 
      and allergic edema of ears in mice. In addition, activated RBL-2H3 cells induced 
      the expression of inflammatory cytokines (tumor necrosis factor-alpha and 
      interleukin-4), which are critical for the pathogenesis of allergic disease, 
      through the activation of c-Jun N-terminal kinase (JNK). Inhibition of JNK 
      activation by hispidulin treatment reduced the induction of cytokine expression 
      in the activated mast cells. Our results indicate that hispidulin might be a 
      possible therapeutic candidate for allergic inflammatory diseases through the 
      suppression of degranulation and inflammatory cytokines expression.
FAU - Kim, Dong Eun
AU  - Kim DE
AD  - Department of Otolaryngology, School of Medicine, Keimyung University, 1095 
      Dalgubeoldaero, Dalseo-Gu, Daegu 42601, Korea. entkde@dsmc.or.kr.
FAU - Min, Kyoung-Jin
AU  - Min KJ
AD  - Department of Immunology, School of Medicine, Keimyung University, 1095 
      Dalgubeoldaero, Dalseo-Gu, Daegu 42601, Korea. kyoungjin.min@gmail.com.
FAU - Kim, Min-Jong
AU  - Kim MJ
AUID- ORCID: 0000-0003-1224-4507
AD  - Department of Pharmacology, CMRI, School of Medicine, Kyungpook National 
      University, Daegu 41944, Korea. wowdamien@hanmail.net.
FAU - Kim, Sang-Hyun
AU  - Kim SH
AD  - Department of Pharmacology, CMRI, School of Medicine, Kyungpook National 
      University, Daegu 41944, Korea. shkim72@knu.ac.kr.
FAU - Kwon, Taeg Kyu
AU  - Kwon TK
AUID- ORCID: 0000-0003-1204-2059
AD  - Department of Immunology, School of Medicine, Keimyung University, 1095 
      Dalgubeoldaero, Dalseo-Gu, Daegu 42601, Korea. kwontk@dsmc.or.kr.
LA  - eng
GR  - 2014R1A5A2010008/National Research Foundation of Korea/
GR  - NRF-2017R1D1A1B03028366/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20190605
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Cytokines)
RN  - 0 (Flavones)
RN  - 0 (Inflammation Mediators)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - N7F61604C2 (hispidulin)
SB  - IM
MH  - Animals
MH  - Cell Degranulation/drug effects
MH  - Cytokines/*metabolism
MH  - *Down-Regulation/drug effects
MH  - Flavones/chemistry/pharmacology/*therapeutic use
MH  - *Histamine Release/drug effects
MH  - Hypersensitivity/complications/*drug therapy
MH  - Immunoglobulin E/metabolism
MH  - Inflammation/complications/*drug therapy/pathology
MH  - Inflammation Mediators/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Male
MH  - Mast Cells/drug effects/*pathology
MH  - Mice, Inbred ICR
MH  - Passive Cutaneous Anaphylaxis/drug effects
MH  - Phosphorylation/drug effects
PMC - PMC6600596
OTO - NOTNLM
OT  - Hispidulin
OT  - allergy
OT  - inflammation
OT  - mast cells
COIS- The authors declare no conflicts of interest.
EDAT- 2019/06/15 06:00
MHDA- 2019/11/30 06:00
PMCR- 2019/06/05
CRDT- 2019/06/15 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/05/24 00:00 [revised]
PHST- 2019/06/04 00:00 [accepted]
PHST- 2019/06/15 06:00 [entrez]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2019/11/30 06:00 [medline]
PHST- 2019/06/05 00:00 [pmc-release]
AID - molecules24112131 [pii]
AID - molecules-24-02131 [pii]
AID - 10.3390/molecules24112131 [doi]
PST - epublish
SO  - Molecules. 2019 Jun 5;24(11):2131. doi: 10.3390/molecules24112131.