PMID- 31196171
OWN - NLM
STAT- MEDLINE
DCOM- 20200615
LR  - 20200615
IS  - 1756-8935 (Electronic)
IS  - 1756-8935 (Linking)
VI  - 12
IP  - 1
DP  - 2019 Jun 13
TI  - Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes 
      progression and metastasis of laryngeal squamous cell carcinoma.
PG  - 35
LID - 10.1186/s13072-019-0283-8 [doi]
LID - 35
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is among the most common 
      malignant tumors with poor prognosis. Accumulating evidences have identified the 
      important roles of long noncoding RNAs (lncRNAs) in the initiation and 
      progression of various cancer types; however, the global lncRNAs expression 
      profile for metastatic LSCC is limited. RESULTS: In the present study, we screen 
      expression profiles of lncRNAs in advanced LSCC patients with paired tumor 
      tissues and corresponding normal tissues by microarrays. We identify numerous 
      differentially expressed transcripts, and after the necessary verification of the 
      transcripts expression in expanded samples, we experimentally validate the 
      expression patterns of the remarkable low expressed gene, SSTR5, and its 
      antisense lncRNA, SSTR5-AS1. Downregulation of SSTR5 is detected in LSCC tissues 
      and laryngeal carcinoma cells. Aberrant DNA hypermethylation of the CpG sites 
      clustered in the exon 1 and accumulation of inactive histone modifications at 
      SSTR5 promoter region may be epigenetic mechanisms for its inactivation in LSCC. 
      SSTR5-AS1 may play antitumor role in LSCC and may be regulated by the 
      hypermethylation of the same CpG sites with SSTR5. SSTR5-AS1 inhibits laryngeal 
      carcinoma cells proliferation, migration, and invasion. SSTR5-AS1 increases the 
      enrichment of MLL3 and H3K4me3 at the promoter region of SSTR5 by interacting 
      with MLL3 and further induces the transcription of SSTR5. Furthermore, SSTR5-AS1 
      interacts with and recruits TET1 to its target gene E-cadherin to activate its 
      expression. CONCLUSION: These findings suggest that the identified lncRNAs and 
      mRNAs may be potential biomarkers in metastatic LSCC, and SSTR5-AS1 may act as a 
      tumor suppressor as well as a potential biomarker for antitumor therapy.
FAU - Wang, Baoshan
AU  - Wang B
AD  - Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei 
      Medical University, Heping West Road 215, Shijiazhuang, 050005, Hebei, China. 
      hebwangbs@163.com.
FAU - Zhao, Lei
AU  - Zhao L
AD  - Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei 
      Medical University, Heping West Road 215, Shijiazhuang, 050005, Hebei, China.
FAU - Chi, Weiwei
AU  - Chi W
AD  - Otolaryngology Head and Neck Surgery Department, The First Hospital of Hebei 
      Medical University, Shijiazhuang, Hebei, China.
FAU - Cao, Huan
AU  - Cao H
AD  - Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei 
      Medical University, Heping West Road 215, Shijiazhuang, 050005, Hebei, China.
FAU - Cui, Weina
AU  - Cui W
AD  - Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei 
      Medical University, Heping West Road 215, Shijiazhuang, 050005, Hebei, China.
FAU - Meng, Wenxia
AU  - Meng W
AD  - Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei 
      Medical University, Heping West Road 215, Shijiazhuang, 050005, Hebei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190613
PL  - England
TA  - Epigenetics Chromatin
JT  - Epigenetics & chromatin
JID - 101471619
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Receptors, Somatostatin)
RN  - 8X85ZJG6XJ (somatostatin receptor 5)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.- (TET1 protein, human)
SB  - IM
MH  - Aged
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *DNA Methylation
MH  - Disease Progression
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Mixed Function Oxygenases/metabolism
MH  - Neoplasm Metastasis
MH  - Oligoribonucleotides, Antisense/*genetics/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - Receptors, Somatostatin/*genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/metabolism/pathology
PMC - PMC6563380
OTO - NOTNLM
OT  - Expression
OT  - Laryngeal squamous cell carcinoma
OT  - Methylation
OT  - SSTR5
OT  - SSTR5-AS1
COIS- The authors declare that they have no competing interests.
EDAT- 2019/06/15 06:00
MHDA- 2020/06/17 06:00
PMCR- 2019/06/13
CRDT- 2019/06/15 06:00
PHST- 2018/11/12 00:00 [received]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/06/15 06:00 [entrez]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/06/13 00:00 [pmc-release]
AID - 10.1186/s13072-019-0283-8 [pii]
AID - 283 [pii]
AID - 10.1186/s13072-019-0283-8 [doi]
PST - epublish
SO  - Epigenetics Chromatin. 2019 Jun 13;12(1):35. doi: 10.1186/s13072-019-0283-8.