PMID- 31196172
OWN - NLM
STAT- MEDLINE
DCOM- 20200529
LR  - 20231012
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 21
IP  - 1
DP  - 2019 Jun 13
TI  - Calorie restriction with regular chow, but not a high-fat diet, delays onset of 
      spontaneous osteoarthritis in the Hartley guinea pig model.
PG  - 145
LID - 10.1186/s13075-019-1925-8 [doi]
LID - 145
AB  - BACKGROUND: Obesity is a leading risk factor for osteoarthritis (OA). In 
      contrast, calorie restriction (CR) may lessen OA due to improved systemic 
      inflammatory status and reduced weight-bearing. The aim of this study was to 
      determine how CR with regular chow versus a high-fat diet (HFD) alters OA 
      progression using the Hartley guinea pig model of disease. METHODS: Twenty-four 
      male guinea pigs were allocated to four groups at 2 months of age: (1) ad libitum 
      regular chow (obese), (2) CR regular chow (lean), (3) ad libitum HFD, and (4) CR 
      HFD. Animals in both HFD groups ate identical amounts and were combined into one 
      HFD group for analyses. At 5 months, hind limbs were harvested for microcomputed 
      tomography (microCT) and histopathologic evaluation of knee OA. Total body, gonad 
      fat, and infrapatellar fat pad (IFP) masses were recorded. IFPs were collected 
      for gene expression analysis. Immunohistochemistry for monocyte chemoattractant 
      protein-1 (MCP-1) was performed on intact joints. Serum was utilized for protein 
      C3 measurement. All data were compared using ordinary one-way ANOVA analyses with 
      Tukey's post-hoc tests. RESULTS: Body mass in the lean and HFD groups were 
      similar and lower than the obese group. Despite this, gonad fat pads in the HFD 
      group were comparable to the obese group. MicroCT and histologic OA scores were 
      similar in obese and HFD groups; both scores were significantly lower in the lean 
      group. Obese and HFD groups displayed increased gene expression of 
      pro-inflammatory and catabolic mediators in IFPs relative to lean animals. 
      Consistent with this, immunohistochemistry for MCP-1 in knee joints demonstrated 
      strong positive staining in obese and HFD groups but was minimally detected in 
      lean animals. Serum protein C3 levels were also statistically higher. 
      CONCLUSIONS: This study demonstrated that CR with a regular chow diet lessened 
      knee OA in the Hartley guinea pig and was associated with decreased local and 
      systemic inflammation compared to obese animals. HFD animals, although under CR 
      conditions, had OA scores and inflammatory markers similar to obese animals. 
      Thus, diet composition, and not solely body weight, may be a key factor in 
      development of OA.
FAU - Radakovich, Lauren B
AU  - Radakovich LB
AD  - Department of Microbiology, Immunology, Pathology, Colorado State University, 200 
      West Lake Street, Fort Collins, CO, 80521, USA.
FAU - Marolf, Angela J
AU  - Marolf AJ
AD  - Department of Environmental and Radiological Health Sciences, Colorado State 
      University, 123 Flint Cancer Center, Fort Collins, CO, 80523, USA.
FAU - Culver, Lauren A
AU  - Culver LA
AD  - Department of Microbiology, Immunology, Pathology, Colorado State University, 200 
      West Lake Street, Fort Collins, CO, 80521, USA.
FAU - Santangelo, Kelly S
AU  - Santangelo KS
AUID- ORCID: 0000-0002-2348-594X
AD  - Department of Microbiology, Immunology, Pathology, Colorado State University, 200 
      West Lake Street, Fort Collins, CO, 80521, USA. Kelly.Santangelo@colostate.edu.
LA  - eng
GR  - P30 CA046934/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190613
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - *Animal Feed
MH  - Animals
MH  - Caloric Restriction/*methods
MH  - Chemokine CCL2/metabolism
MH  - Diet, High-Fat/*adverse effects
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Guinea Pigs
MH  - Knee Joint/*diagnostic imaging/metabolism
MH  - Male
MH  - Osteoarthritis, Knee/diagnosis/*diet therapy/physiopathology
MH  - Weight-Bearing/physiology
MH  - X-Ray Microtomography
PMC - PMC6567638
OTO - NOTNLM
OT  - Calorie restriction
OT  - Hartley guinea pig
OT  - High-fat diet
OT  - Obesity
OT  - Osteoarthritis
COIS- The authors declare that they have no competing interests.
EDAT- 2019/06/15 06:00
MHDA- 2020/05/30 06:00
PMCR- 2019/06/13
CRDT- 2019/06/15 06:00
PHST- 2018/11/02 00:00 [received]
PHST- 2019/05/23 00:00 [accepted]
PHST- 2019/06/15 06:00 [entrez]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2020/05/30 06:00 [medline]
PHST- 2019/06/13 00:00 [pmc-release]
AID - 10.1186/s13075-019-1925-8 [pii]
AID - 1925 [pii]
AID - 10.1186/s13075-019-1925-8 [doi]
PST - epublish
SO  - Arthritis Res Ther. 2019 Jun 13;21(1):145. doi: 10.1186/s13075-019-1925-8.