PMID- 31197672
OWN - NLM
STAT- MEDLINE
DCOM- 20191003
LR  - 20200225
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 26
IP  - 23
DP  - 2019 Aug
TI  - Competitive immunoassay for simultaneous detection of imidacloprid and 
      thiacloprid by upconversion nanoparticles and magnetic nanoparticles.
PG  - 23471-23479
LID - 10.1007/s11356-019-05635-8 [doi]
AB  - A rapid and sensitive immunoassay for the simultaneous detection of imidacloprid 
      and thiacloprid was developed by using magnetic nanoparticles (MNPs) and 
      upconversion nanoparticles (UCNPs). The UCNPs of NaYF(4):Yb, Er and NaYF(4):Yb, 
      Tm were synthesized and conjugated with anti-imidacloprid monoclonal antibody 
      (mAb) and anti-thiacloprid mAb as signal labels, while the MNPs were conjugated 
      with antigens of thiacloprid and imidacloprid as separation elements. The 
      fluorescence intensities of Yb/Er- and Yb/Tm-doped UCNPs were detected 
      simultaneously in 544 nm and 477 nm under the excitation of NIR light (980 nm). 
      The amounts of mAb-conjugated UCNPs that were separated by antigen-conjugated 
      MNPs were determined based on competitive immunoassays. Under the optimal 
      conditions, the 50% inhibiting concentration (IC(50)) and limit of detection 
      (LOD, IC(10)) were 5.80 and 0.32 ng/mL for imidacloprid and 6.45 and 0.61 ng/mL 
      for thiacloprid, respectively. The immunoassay exhibited negligible 
      cross-reactivity with analogs of imidacloprid and thiacloprid except imidaclothiz 
      (86.2%). The average recoveries of imidacloprid and thiacloprid in environmental 
      and agricultural samples, including paddy water, soil, pears, oranges, cucumbers, 
      and wheat, ranged from 78.4 to 105.9% with relative standard deviations (RSDs) of 
      2.1-11.9% for imidacloprid and ranged from 82.5 to 102.3% with RSDs of 1.0-16.5% 
      for thiacloprid. In addition, the results of the immunoassay correlated well with 
      high-performance liquid chromatography for the detection of the authentic 
      samples.
FAU - Tao, Zhexuan
AU  - Tao Z
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Deng, Jiaqi
AU  - Deng J
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Chen, He
AU  - Chen H
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Ding, Yuan
AU  - Ding Y
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Hua, Xiude
AU  - Hua X
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China.
FAU - Wang, Minghua
AU  - Wang M
AUID- ORCID: 0000-0001-5715-4981
AD  - College of Plant Protection, State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application, Nanjing Agricultural University, 
      Nanjing, Jiangsu Province, China. wangmha@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190614
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Neonicotinoids)
RN  - 0 (Nitro Compounds)
RN  - 0 (Thiazines)
RN  - 0 (Thiazoles)
RN  - 3BN7M937V8 (imidacloprid)
RN  - DSV3A944A4 (thiacloprid)
RN  - E070T0J4TU (imidaclothiz)
SB  - IM
MH  - Fluorescence
MH  - Immunoassay/*methods
MH  - Magnetics
MH  - Magnetite Nanoparticles/*chemistry
MH  - Nanoparticles/chemistry
MH  - Neonicotinoids/*analysis
MH  - Nitro Compounds/*analysis
MH  - Thiazines/*analysis
MH  - Thiazoles
OTO - NOTNLM
OT  - Imidacloprid
OT  - Magnetic nanoparticles (MNPs)
OT  - Simultaneous detection
OT  - Thiacloprid
OT  - Upconversion nanoparticles (UCNPs)
EDAT- 2019/06/15 06:00
MHDA- 2019/10/08 06:00
CRDT- 2019/06/15 06:00
PHST- 2019/01/30 00:00 [received]
PHST- 2019/05/29 00:00 [accepted]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2019/06/15 06:00 [entrez]
AID - 10.1007/s11356-019-05635-8 [pii]
AID - 10.1007/s11356-019-05635-8 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2019 Aug;26(23):23471-23479. doi: 
      10.1007/s11356-019-05635-8. Epub 2019 Jun 14.