PMID- 31197771
OWN - NLM
STAT- MEDLINE
DCOM- 20200324
LR  - 20200324
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2017
DP  - 2019
TI  - Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant.
PG  - 97-107
LID - 10.1007/978-1-4939-9574-5_8 [doi]
AB  - In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) 
      microenvironment in the bone marrow-the so-called stem cell niche-by flooding the 
      niche with clonal progeny of leukemic cells. They can exploit signaling pathways 
      which are critical for HSC development to support their own survival, homing, and 
      maintenance. These interactions of leukemic cells with the microenvironment have 
      an impact on therapy progress and patient outcome. Therefore, signals for homing 
      and anchorage of leukemic cells to the bone marrow have to be investigated by 
      using tools that allow the migration of cells toward critical signals. Here, we 
      describe an in vitro migration assay for leukemic cells toward a chemoattractant 
      in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC 
      motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide 
      is filled with a hydrogel system mimicking the extracellular matrix in vivo. The 
      cells are encapsulated into the hydrogel network during polymerization, and a 
      CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell 
      migration in the 3D network of the hydrogel is monitored by time-lapse 
      microscopy. We describe the experimental setup and the tools for cell tracking 
      and data analysis.
FAU - Zippel, Sabrina
AU  - Zippel S
AD  - Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 
      Eggenstein-Leopoldshafen, Germany.
AD  - Institute of Cell Biology and Biophysics, Leibniz University Hannover, Hannover, 
      Germany.
FAU - Raic, Annamarija
AU  - Raic A
AD  - Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 
      Eggenstein-Leopoldshafen, Germany.
AD  - Institute of Cell Biology and Biophysics, Leibniz University Hannover, Hannover, 
      Germany.
FAU - Lee-Thedieck, Cornelia
AU  - Lee-Thedieck C
AD  - Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 
      Eggenstein-Leopoldshafen, Germany. lee-thedieck@cell.uni-hannover.de.
AD  - Institute of Cell Biology and Biophysics, Leibniz University Hannover, Hannover, 
      Germany. lee-thedieck@cell.uni-hannover.de.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Cell Line, Tumor/*cytology/metabolism
MH  - Cell Movement
MH  - Cell Tracking
MH  - Chemokine CXCL12/*metabolism
MH  - Chemotaxis
MH  - Humans
MH  - Hydrogels/chemistry
MH  - Leukemia/*pathology
MH  - Stem Cell Niche
MH  - Tumor Microenvironment
OTO - NOTNLM
OT  - 3D matrix
OT  - CXCR4/CXCL12 axis
OT  - Chemokine gradient
OT  - Leukemic cells
OT  - Migration
OT  - mu-Slides
EDAT- 2019/06/15 06:00
MHDA- 2020/03/25 06:00
CRDT- 2019/06/15 06:00
PHST- 2019/06/15 06:00 [entrez]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2020/03/25 06:00 [medline]
AID - 10.1007/978-1-4939-9574-5_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;2017:97-107. doi: 10.1007/978-1-4939-9574-5_8.