PMID- 31198002
OWN - NLM
STAT- MEDLINE
DCOM- 20190830
LR  - 20220410
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 33
IP  - 6
DP  - 2019 Jun 15
TI  - [Mechanism of p38 mitogen activated protein kinase signaling pathway on promoting 
      the hypertrophy of human lumbar ligamentum flavum via transforming growth factor 
      beta (1)/connective tissue growth factor].
PG  - 730-735
LID - 10.7507/1002-1892.201811140 [doi]
AB  - OBJECTIVE: To investigate the mechanism of p38 mitogen activated protein kinase 
      (MAPK) signaling pathway in regulating the hyperplasia and hypertrophy of human 
      lumbar ligamentum flavum via transforming growth factor beta (1) (TGF-beta 
      (1))/connective tissue growth factor (CTGF). METHODS: The lumbar ligamentum 
      flavum tissue taken from patient with lumbar intervertebral disc herniation was 
      isolated by collagenase-predigested explant cultures. The ligamentum flavum cells 
      were treated with the extracellular regulated protein kinase pathway blocker 
      PD98059, c-Jun N-terminal kinase pathway blocker SP600125, and p38 pathway 
      blocker SB203580, and then the mRNA expressions of CTGF, collagen type Ⅰ, and 
      collagen type Ⅲ were detected by real-time fluorescence quantitative PCR 
      (qRT-PCR). The ligamentum flavum cells were divided into 4 groups, and 
      transfected with small interfering RNA (siRNA), p38 siRNA, siRNA+3 ng/mL TGF-beta 
      (1), and p38 siRNA+3 ng/mL TGF-beta (1) in groups A, B, C, and D, respectively. 
      After 24 hours of transfection, immunofluorescence staining was performed to 
      observe the expressions of p38 and phosphorylation p38 (p-p38); the relative mRNA 
      expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ in each group were 
      detected by qRT-PCR; the protein expression of CTGF in each group was detected by 
      Western blot. RESULTS: p38 pathway blocker SB203580 could significantly reduce 
      the relative mRNA expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ ( 
      P<0.05). After 24 hours of transfection, immunofluorescence staining showed 
      positive staining with p38 and p-p38 expressions in groups A, C, and D and 
      negative staining in group B. Compared with group A, the relative mRNA 
      expressions of CTGF, collagen type Ⅰ, and collagen type Ⅲ and relative protein 
      expression of CTGF in group B decreased significantly ( P<0.05), while those in 
      groups C and D increased significantly ( P<0.05); and those indicators 
      significantly increased in group C than in group D ( P<0.05). CONCLUSION: TGF-beta 
      (1)/CTGF based on the p38 MAPK signaling pathway play an important role in the 
      occurance and development of hypertrophy of human lumbar ligamentum flavum.
FAU - Lu, Changhuai
AU  - Lu C
AD  - Department of Spine Disease Area of Orthopedics and Traumatology, No.1 
      Traditional Chinese Medicine Hospital of Changde, Changde Hospital Affiliated to 
      Hunan University of Traditional Chinese Medicine, Changde Hunan, 415000, 
      P.R.China.
FAU - Liu, Zhijun
AU  - Liu Z
AD  - Department of Spine Disease Area of Orthopedics and Traumatology, No.1 
      Traditional Chinese Medicine Hospital of Changde, Changde Hospital Affiliated to 
      Hunan University of Traditional Chinese Medicine, Changde Hunan, 415000, 
      P.R.China.
FAU - Zhang, Hongbo
AU  - Zhang H
AD  - Department of Spine Disease Area of Orthopedics and Traumatology, No.1 
      Traditional Chinese Medicine Hospital of Changde, Changde Hospital Affiliated to 
      Hunan University of Traditional Chinese Medicine, Changde Hunan, 415000, 
      P.R.China.
FAU - Duan, Yang
AU  - Duan Y
AD  - Department of Spinal Surgery, Zhujiang Hospital of Southern Medical University, 
      Guangzhou Guangdong, 510282, P.R.China.duanyang2011@163.com.
FAU - Cao, Yanlin
AU  - Cao Y
AD  - Department of Spinal Surgery, Zhujiang Hospital of Southern Medical University, 
      Guangzhou Guangdong, 510282, P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (CCN2 protein, human)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Cells, Cultured
MH  - *Connective Tissue Growth Factor/physiology
MH  - Humans
MH  - Hypertrophy
MH  - *Ligamentum Flavum/metabolism
MH  - *Signal Transduction
MH  - Transforming Growth Factor beta
MH  - Transforming Growth Factor beta1/physiology
MH  - *p38 Mitogen-Activated Protein Kinases/physiology
PMC - PMC8355761
OTO - NOTNLM
OT  - Mitogen activated protein kinase
OT  - connective tissue growth factor
OT  - ligamentum flavum
OT  - transforming growth factor beta1
EDAT- 2019/06/15 06:00
MHDA- 2019/08/31 06:00
PMCR- 2019/06/01
CRDT- 2019/06/15 06:00
PHST- 2019/06/15 06:00 [entrez]
PHST- 2019/06/15 06:00 [pubmed]
PHST- 2019/08/31 06:00 [medline]
PHST- 2019/06/01 00:00 [pmc-release]
AID - zgxfcjwkzz-33-6-730 [pii]
AID - 10.7507/1002-1892.201811140 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019 Jun 15;33(6):730-735. doi: 
      10.7507/1002-1892.201811140.