PMID- 31201457 OWN - NLM STAT- MEDLINE DCOM- 20191213 LR - 20191217 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) VI - 411 IP - 22 DP - 2019 Sep TI - Quantitative monitoring of a panel of stress-induced biomarkers in human plasma by liquid chromatography-tandem mass spectrometry: an application in a comparative study between depressive patients and healthy subjects. PG - 5765-5777 LID - 10.1007/s00216-019-01956-2 [doi] AB - Using a metabolomic approach, we have found that stress can induce oxidative damage by disturbing the creatine/phosphocreatine shuttle system and purinergic pathway, leading to an excessive membrane breakdown. To further validate our findings and to monitor the biological impact of stress in research of clinical psychiatry, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine a panel of biomarkers comprising choline, creatine, purinergic metabolites, neurosteroids, lysophosphatidylcholines, and phosphatidylethanolamines in human plasma. After optimization of the extraction protocol, all the 15 analytes plus 4 internal standards with distinct polarities were extracted into an organic phase using methyl tert-butyl ether/methanol (1:1, v/v). A reversed-phase C8 column under gradient elution consisting of aqueous phase A of 5 mM ammonium acetate buffer solution containing 0.1% formic acid and organic phase B of acetonitrile/2-propanol (3:7, v/v) was utilized for separation. Four sequential periods under positive or negative ion mode were combined for the determination of analytes with specific multiple reaction monitoring transitions. For all analytes, this method exhibited good linearity with coefficients of determination (R(2)) higher than 0.99. The lower limit of quantification (LLOQ) values ranged from 0.05 to 80.0 ng/mL. Recovery between 70.5 and 97.3% was obtained by spiking standards to plasma samples stripped by powdered activated carbon. The intra- and inter-assay relative standard deviations (RSDs) of the analyses varied between 2.0 and 13.3%. The mean accuracy ranged from 90.6 to 109.0%. The matrix effect ranged from 91.2 to 107.3% with variations less than 9.0%. Stability under different conditions was tested, with mean recoveries varying between 90.4 and 109.7%. Finally, the established method was successfully applied to analyze the plasma samples from a small cohort of 30 patients with major depressive disorder and 30 matched healthy controls. Graphical abstract. FAU - Cai, HuaLin AU - Cai H AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. hualincai@csu.edu.cn. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. hualincai@csu.edu.cn. FAU - Cao, Ting AU - Cao T AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Li, NaNa AU - Li N AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Fang, PingFei AU - Fang P AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Xu, Ping AU - Xu P AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Wu, XiangXin AU - Wu X AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Zhang, BiKui AU - Zhang B AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. FAU - Xiang, DaXiong AU - Xiang D AD - Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. xiangdaxiong@csu.edu.cn. AD - Institute of Clinical Pharmacy, Central South University, Changsha, 410011, Hunan, China. xiangdaxiong@csu.edu.cn. LA - eng GR - NSFC81401113/Nature Science Foundation of China/ GR - 2017JJ3444/Hunan Provincial Natural Science Foundation of China/ PT - Journal Article DEP - 20190614 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 RN - 0 (Biomarkers) SB - IM MH - Biomarkers/blood MH - Case-Control Studies MH - Chromatography, Liquid/*methods MH - Depression/*blood MH - Humans MH - Reproducibility of Results MH - Tandem Mass Spectrometry/*methods OTO - NOTNLM OT - Liquid chromatography OT - Neurosteroids OT - Phospholipids OT - Purines OT - Stress OT - Tandem mass spectrometry EDAT- 2019/06/16 06:00 MHDA- 2019/12/18 06:00 CRDT- 2019/06/16 06:00 PHST- 2019/03/23 00:00 [received] PHST- 2019/05/29 00:00 [accepted] PHST- 2019/05/21 00:00 [revised] PHST- 2019/06/16 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2019/06/16 06:00 [entrez] AID - 10.1007/s00216-019-01956-2 [pii] AID - 10.1007/s00216-019-01956-2 [doi] PST - ppublish SO - Anal Bioanal Chem. 2019 Sep;411(22):5765-5777. doi: 10.1007/s00216-019-01956-2. Epub 2019 Jun 14.