PMID- 31201733
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20200614
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 34
IP  - 4
DP  - 2019 Aug
TI  - Improving Baculovirus Transduction of Mammalian Cells by Incorporation of 
      Thogotovirus Glycoproteins.
PG  - 454-466
LID - 10.1007/s12250-019-00133-0 [doi]
AB  - Baculovirus can transduce a wide range of mammalian cells and is considered a 
      promising gene therapy vector. However, the low transduction efficiency of 
      baculovirus into many mammalian cells limits its practical application. 
      Co-expressing heterologous viral glycoproteins (GPs), such as vesicular 
      stomatitis virus G protein (VSV G), with baculovirus native envelope protein GP64 
      is one of the feasible strategies for improving virus transduction. Tick-borne 
      thogotoviruses infect mammals and their GPs share sequence/structure homology and 
      common evolutionary origins with baculovirus GP64. Herein, we tested whether 
      thogotovirus GPs could facilitate the entry of the prototype baculovirus 
      Autographa californica multiple multiple nucleopolyhedrovirus (AcMNPV) into 
      mammalian cells. The gp genes of two thogotoviruses, Thogoto virus and Dhori 
      virus, were inserted into the AcMNPV genome. Both GPs were properly expressed and 
      incorporated into the envelope of the recombinant AcMNPVs. The transduction rates 
      of recombinant AcMNPVs expressing the two thogotovirus GPs increased for 
      approximately 4-12 fold compared to the wild type AcMNPV in six of the 12 tested 
      mammalian cell lines. It seemed that thogotovirus GPs provide the recombinant 
      AcMNPVs with different cell tropisms and showed better performance in several 
      mammalian cells compared to VSV G incorporated AcMNPV. Further studies showed 
      that the improved transduction was a result of augmented virus-endosome fusion 
      and endosome escaping, rather than increased cell binding or internalization. We 
      found the AcMNPV envelope protein GP64-mediated fusion was enhanced by the 
      thogotovirus GPs at relatively higher pH conditions. Therefore, the thogotovirus 
      GPs represent novel candidates to improve baculovirus-based gene delivery 
      vectors.
FAU - Hu, Liangbo
AU  - Hu L
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China.
AD  - University of the Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Li, Yimeng
AU  - Li Y
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China.
AD  - University of the Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Deng, Fei
AU  - Deng F
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China.
FAU - Hu, Zhihong
AU  - Hu Z
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China.
FAU - Wang, Hualin
AU  - Wang H
AUID- ORCID: 0000-0001-8916-4578
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China. h.wang@wh.iov.cn.
FAU - Wang, Manli
AU  - Wang M
AUID- ORCID: 0000-0001-8701-3530
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, 430071, China. wangml@wh.iov.cn.
LA  - eng
PT  - Journal Article
DEP - 20190614
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
RN  - 0 (G protein, vesicular stomatitis virus)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Fusion Proteins)
RN  - Autographa californica multiple nuclear polyhedrosis virus
SB  - IM
MH  - Animals
MH  - Baculoviridae/*genetics
MH  - Cell Line
MH  - Genetic Vectors
MH  - Genome, Viral
MH  - Glycoproteins/*genetics
MH  - Humans
MH  - Membrane Glycoproteins/genetics
MH  - Nucleopolyhedroviruses/physiology
MH  - Recombinant Proteins/genetics
MH  - Thogotovirus/*genetics
MH  - *Transduction, Genetic
MH  - Viral Envelope Proteins/genetics
MH  - Viral Fusion Proteins/genetics
MH  - Viral Tropism
MH  - *Virus Internalization
PMC - PMC6687793
OTO - NOTNLM
OT  - Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
OT  - Baculovirus
OT  - Glycoprotein
OT  - Mammalian cells
OT  - Thogotovirus
OT  - Transduction
COIS- The authors declare no competing interests.
EDAT- 2019/06/16 06:00
MHDA- 2019/12/18 06:00
PMCR- 2020/06/14
CRDT- 2019/06/16 06:00
PHST- 2019/01/20 00:00 [received]
PHST- 2019/04/09 00:00 [accepted]
PHST- 2019/06/16 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/06/16 06:00 [entrez]
PHST- 2020/06/14 00:00 [pmc-release]
AID - 10.1007/s12250-019-00133-0 [pii]
AID - 133 [pii]
AID - 10.1007/s12250-019-00133-0 [doi]
PST - ppublish
SO  - Virol Sin. 2019 Aug;34(4):454-466. doi: 10.1007/s12250-019-00133-0. Epub 2019 Jun 
      14.