PMID- 31202458
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20200713
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 516
IP  - 1
DP  - 2019 Aug 13
TI  - DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells 
      via Wnt/beta-catenin pathway mediated histone methylation of DKK4.
PG  - 171-176
LID - S0006-291X(19)31152-0 [pii]
LID - 10.1016/j.bbrc.2019.06.029 [doi]
AB  - OBJECTIVE: Distal-less homeobox 3 (DLX3) is an important transcription factor 
      involved in the osteogenic differentiation of bone marrow mesenchymal stem cells 
      (BMSCs). However, the underlying mechanism is not clear. This study investigated 
      the underlying mechanism of DLX3 in osteogenic differentiation. METHODS: DLX3 
      overexpression and knockdown in cells were achieved using lentiviruses. The 
      osteogenic differentiation of BMSCs was detected using alkaline phosphatase 
      expression, alizarin red staining, real-time quantitative polymerase chain 
      reaction (RT-qPCR), Western blotting, and chromatin immunoprecipitation (ChIP) 
      assays. RESULTS: DLX3 overexpression promoted the osteogenic differentiation of 
      BMSCs, whereas DLX3 knockdown reduced the osteogenic differentiation of BMSCs. 
      RT-qPCR and Western blotting assays showed that DLX3 modulated osteogenic 
      differentiation via the Wnt/beta-catenin pathway. ChIP-qPCR showed that DLX3 
      knockdown promoted DKK4 expression by decreasing the enrichment of histone H3 
      lysine 27 trimethylation (H3K27me3) in the promotor region of DKK4. CONCLUSION: 
      Our data implied that DLX3 regulated Wnt/beta-catenin pathway through histone 
      modification of DKK4 during the osteogenic differentiation of BMSCs.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Sun, Shichen
AU  - Sun S
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China.
FAU - Yu, Miao
AU  - Yu M
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China.
FAU - Fan, Zhuangzhuang
AU  - Fan Z
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China.
FAU - Yeh, I-Ting
AU  - Yeh IT
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China.
FAU - Feng, Hailan
AU  - Feng H
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China.
FAU - Liu, Haochen
AU  - Liu H
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China. Electronic address: 
      talentlhc@163.com.
FAU - Han, Dong
AU  - Han D
AD  - Department of Prosthodontics, Peking University School and Hospital of 
      Stomatology, National Clinical Research Center for Oral Diseases, National 
      Engineering Laboratory for Digital and Material Technology of Stomatology, 
      Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, 
      Haidian District, Beijing, 100081, PR China. Electronic address: 
      donghan@bjmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190613
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DKK4 protein, human)
RN  - 0 (Distal-less homeobox proteins)
RN  - 0 (Histones)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Histones/*metabolism
MH  - Homeodomain Proteins/*metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*metabolism
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Methylation
MH  - *Osteogenesis
MH  - Transcription Factors/*metabolism
MH  - *Wnt Signaling Pathway
OTO - NOTNLM
OT  - DKK4
OT  - DLX3
OT  - Epigenetic regulation
OT  - Histone methylation
OT  - Osteogenic differentiation
EDAT- 2019/06/17 06:00
MHDA- 2020/07/14 06:00
CRDT- 2019/06/17 06:00
PHST- 2019/06/01 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/17 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2019/06/17 06:00 [entrez]
AID - S0006-291X(19)31152-0 [pii]
AID - 10.1016/j.bbrc.2019.06.029 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2019 Aug 13;516(1):171-176. doi: 
      10.1016/j.bbrc.2019.06.029. Epub 2019 Jun 13.