PMID- 31202965
OWN - NLM
STAT- MEDLINE
DCOM- 20191202
LR  - 20191202
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 92
DP  - 2019 Sep
TI  - Functional characterization of three fish-specific interleukin-23 isoforms as 
      regulators of Th17 signature cytokine expression in grass carp head kidney 
      leukocytes.
PG  - 315-321
LID - S1050-4648(19)30675-8 [pii]
LID - 10.1016/j.fsi.2019.06.028 [doi]
AB  - Mammalian Interleukin (IL)-23 is a heterodimeric cytokine with an IL-23-specific 
      P19 subunit and a P40 subunit shared with IL-12, and plays a key role in the 
      regulation of cell differentiation as well as inflammation. We previously 
      demonstrated the existence of three soluble fish Interleukin (Il)-23 isoforms 
      consist of a single P19 and one of three P40 isoforms (P40a/b/c) in grass carp. 
      In the present study, three recombinant grass carp Il-23 (rgcIl-23) isoforms were 
      prepared by linking gcP19 and gcP40a/b/c in a prokaryotic expression system, and 
      then their functional properties were verified in grass carp head kidney 
      leukocytes (HKLs). All three rgcIl-23 isoforms showed the bioactivities to 
      divergently upregulate the mRNA expression of Th17 signature cytokines (il17a/f1, 
      il21, il22 and il26) as well as Il-23 receptor (il23r) in HKLs. Moreover, they 
      also promoted gcIl-17a/f1 secretion in a dose-dependent manner, strengthening 
      their roles in Th17-like response. Furthermore, induction of il17a/f1 and il23r 
      transcription by rgcIl-23 was blocked by a STAT3 inhibitor in grass carp HKLs, 
      suggesting the involvement of STAT3 signaling in these inductions. Taken 
      together, we for the first time identified the bioactivities of fish Il-23 
      isoforms and particularly revealed the existence of Il-23/Il-17a/f1 axis in fish, 
      thereby advancing our understanding of Th17-like responses in fish immunity.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Yin, Licheng
AU  - Yin L
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Ren, Jingqi
AU  - Ren J
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Dan
AU  - Wang D
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Feng, Shiyu
AU  - Feng S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Qiu, Xinyang
AU  - Qiu X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Lv, Mengyuan
AU  - Lv M
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China. Electronic address: 
      zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190613
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-23)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Carps/*genetics/*immunology
MH  - Cytokines/genetics/immunology
MH  - Fish Proteins/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - Head Kidney/immunology
MH  - Interleukin-23/*genetics/metabolism
MH  - Leukocytes/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins/genetics/immunology
MH  - Signal Transduction/immunology
MH  - Th17 Cells/*immunology
OTO - NOTNLM
OT  - Functional identification
OT  - Grass carp
OT  - Il-17a/f1
OT  - Il-23
OT  - Il-23r
OT  - Isoforms
OT  - Th17-like response
EDAT- 2019/06/17 06:00
MHDA- 2019/12/04 06:00
CRDT- 2019/06/17 06:00
PHST- 2019/03/25 00:00 [received]
PHST- 2019/06/12 00:00 [revised]
PHST- 2019/06/12 00:00 [accepted]
PHST- 2019/06/17 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2019/06/17 06:00 [entrez]
AID - S1050-4648(19)30675-8 [pii]
AID - 10.1016/j.fsi.2019.06.028 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Sep;92:315-321. doi: 10.1016/j.fsi.2019.06.028. Epub 
      2019 Jun 13.