PMID- 31203090 OWN - NLM STAT- MEDLINE DCOM- 20200106 LR - 20200106 IS - 1879-3231 (Electronic) IS - 0093-691X (Linking) VI - 135 DP - 2019 Sep 1 TI - Use of color-Doppler ultrasonography for selection of recipients in timed-embryo transfer programs in beef cattle. PG - 73-79 LID - S0093-691X(19)30227-4 [pii] LID - 10.1016/j.theriogenology.2019.06.006 [doi] AB - We aimed to study the association between CL characteristics assessed by color-Doppler ultrasonography (Doppler-US) at the time of embryo transfer (ET) and pregnancy rate (P/ET) in beef recipients. Estrous cycles of crossbred beef recipients were synchronized for timed-ET. On the day of ET (Day 7), CL area, proportion of luteal blood perfusion (BP), and the relationship between the largest dominant follicle (DF) and CL (ipsilateral or contralateral) were determined. Animals (n = 444) received an in vitro produced embryo from Nelore donors, placed in the uterine horn ipsilateral to the CL. Recipients were split retrospectively in three subgroups according to CL area [small (<3 cm(2)), medium (3-4 cm(2)), or large (>4 cm(2))] and three subgroups according to luteal signals of BP [low (/=55%)]. Pregnancy was detected on Days 30-45 by transrectal ultrasonography and P/ET was analyzed considering the effects of cow's category (suckling or non-suckling), CL area, luteal BP and side of DF. P/ET increased along with BP category [low, 45.9%(B), (62/135); medium, 54.1%(AB) (93/172); and high, 58.4%(A) (80/137)]. When luteal BP was evaluated as a continuous variable, a significant (P < 0.05) linear and positive effect was observed on P/ET. A greater (P < 0.05) CL area and serum progesterone concentrations were observed in the medium and high BP than in the low BP category. Although an effect of luteal size category was not significant on P/ET [small, 49% (76/155); medium, 59.7% (83/139); and large, 50.7% (75/148); P > 0.1], when CL area was evaluated as a continuous variable, a quadratic effect (P < 0.05) indicated a positive relationship between P/ET and CL area until luteal tissue reached 4.07 cm(2), followed by a negative relationship. The location of the first-wave DF in relation to the CL did not affect P/ET (P > 0.1). In conclusion, Doppler-US is an innovative tool that has the potential to be used for selection of suitable embryo recipients based on luteal BP. Selection of recipients that have a greater chance of maintaining pregnancy will increase the success of timed-ET programs. CI - Copyright (c) 2019. Published by Elsevier Inc. FAU - Pugliesi, Guilherme AU - Pugliesi G AD - Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil. Electronic address: gpugliesi@usp.br. FAU - Dalmaso de Melo, Gabriela AU - Dalmaso de Melo G AD - Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil. FAU - Silva, Julio Barboza AU - Silva JB AD - Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil; Embryo SYS, Ouro Fino, MG, Brazil. FAU - Carvalhedo, Alexandre Sardinha AU - Carvalhedo AS AD - Embriotec Reproducao Animal, Anapolis, GO, Brazil. FAU - Lopes, Everton AU - Lopes E AD - Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil. FAU - de Siqueira Filho, Emivaldo AU - de Siqueira Filho E AD - Embriotec Reproducao Animal, Anapolis, GO, Brazil. FAU - Silva, Luciano Andrade AU - Silva LA AD - Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, Sao Paulo, Brazil. FAU - Binelli, Mario AU - Binelli M AD - Department of Animal Sciences, University of Florida, Gainesville, FL, USA. LA - eng PT - Journal Article DEP - 20190607 PL - United States TA - Theriogenology JT - Theriogenology JID - 0421510 RN - 4G7DS2Q64Y (Progesterone) SB - IM MH - Animals MH - Cattle/*physiology MH - Embryo Transfer/*veterinary MH - Female MH - Ovary/anatomy & histology/physiology MH - Pregnancy MH - Pregnancy Rate MH - Progesterone/blood MH - Ultrasonography, Doppler, Color/*veterinary OTO - NOTNLM OT - Blood perfusion OT - Cattle OT - Corpus luteum OT - Follicle OT - Pregnancy success EDAT- 2019/06/17 06:00 MHDA- 2020/01/07 06:00 CRDT- 2019/06/17 06:00 PHST- 2019/02/12 00:00 [received] PHST- 2019/05/31 00:00 [revised] PHST- 2019/06/05 00:00 [accepted] PHST- 2019/06/17 06:00 [pubmed] PHST- 2020/01/07 06:00 [medline] PHST- 2019/06/17 06:00 [entrez] AID - S0093-691X(19)30227-4 [pii] AID - 10.1016/j.theriogenology.2019.06.006 [doi] PST - ppublish SO - Theriogenology. 2019 Sep 1;135:73-79. doi: 10.1016/j.theriogenology.2019.06.006. Epub 2019 Jun 7.