PMID- 31204163
OWN - NLM
STAT- MEDLINE
DCOM- 20200403
LR  - 20200403
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 47
IP  - 6
DP  - 2019 Nov-Dec
TI  - Budesonide up-regulates vitamin D receptor expression in human bronchial 
      fibroblasts and enhances the inhibitory effect of calcitriol on airway 
      remodeling.
PG  - 585-590
LID - S0301-0546(19)30052-7 [pii]
LID - 10.1016/j.aller.2019.05.001 [doi]
AB  - INTRODUCTION AND OBJECTIVES: Transforming growth factor beta1 (TGFbeta1) and 
      dysregulated microRNA-21 (miR-21) expression is associated with TGFbeta/Smad 
      signaling pathway activation and fibrosis. While calcitriol has been shown to 
      improve airway remodeling in asthmatic mice, its mechanism remains unknown. In 
      this study, the effect of calcitriol on the TGFbeta/Smad signaling pathway and 
      miR-21 expression in human bronchial fibroblasts was investigated to explore the 
      mechanism of action of calcitriol and the inhaled glucocorticoid, budesonide, in 
      airway remodeling. MATERIALS AND METHODS: Human bronchial fibroblasts were 
      pretreated with budesonide, calcitriol, or budesonide plus calcitriol, and 
      stimulated with TGFbeta1 for 48h. Quantitative real-time PCR was used to determine 
      the expression of miR-21. Western blot was used to determine airway 
      remodeling-related proteins, TGFbeta/Smad signaling pathway-related proteins, 
      glucocorticoid receptor, and vitamin D receptor (VDR) expression. RESULTS: Both 
      budesonide and calcitriol down-regulated miR-21 expression in human bronchial 
      fibroblasts, up-regulated Smad7 expression, and inhibited the expression of 
      airway remodeling-related proteins. Both budesonide and calcitriol up-regulated 
      the low expression of VDR induced by TGFbeta1 in human bronchial fibroblasts. The 
      expression of VDR in the combined treatment group (budesonide plus calcitriol) 
      was significantly higher than that in the calcitriol treatment group. The 
      expression of collagen type I in the combined treatment group was significantly 
      lower than that in the calcitriol treatment group. CONCLUSIONS: Calcitriol can 
      up-regulate the expression of VDR in human bronchial fibroblasts and exert an 
      anti-airway remodeling effect. Budesonide can up-regulate the expression of VDR 
      in human bronchial fibroblasts and enhance the inhibitory effect of calcitriol on 
      airway remodeling.
CI  - Copyright (c) 2019. Published by Elsevier Espana, S.L.U.
FAU - Xu, Yaqin
AU  - Xu Y
AD  - Department of Pediatrics, Wuxi Children's Hospital Affiliated to Nanjing Medical 
      University, Wuxi, Jiangsu, China.
FAU - Qian, Jun
AU  - Qian J
AD  - Department of Pediatrics, Wuxi Children's Hospital Affiliated to Nanjing Medical 
      University, Wuxi, Jiangsu, China.
FAU - Yu, Zhiwei
AU  - Yu Z
AD  - Department of Pediatrics, Wuxi Children's Hospital Affiliated to Nanjing Medical 
      University, Wuxi, Jiangsu, China. Electronic address: yu98204@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20190614
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (SMAD7 protein, human)
RN  - 0 (Smad Proteins)
RN  - 0 (Smad7 Protein)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 51333-22-3 (Budesonide)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Airway Remodeling/drug effects
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Asthma/drug therapy/*immunology
MH  - Bronchi/*pathology
MH  - Budesonide/*pharmacology
MH  - Calcitriol/*pharmacology
MH  - Cells, Cultured
MH  - Drug Synergism
MH  - Fibroblasts/*physiology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - MicroRNAs/genetics
MH  - Receptors, Calcitriol/*metabolism
MH  - Signal Transduction
MH  - Smad Proteins/metabolism
MH  - Smad7 Protein/genetics/metabolism
MH  - Transforming Growth Factor beta1/metabolism
OTO - NOTNLM
OT  - Airway remodeling
OT  - Budesonide
OT  - Calcitriol
EDAT- 2019/06/18 06:00
MHDA- 2020/04/04 06:00
CRDT- 2019/06/18 06:00
PHST- 2018/12/26 00:00 [received]
PHST- 2019/05/02 00:00 [revised]
PHST- 2019/05/06 00:00 [accepted]
PHST- 2019/06/18 06:00 [pubmed]
PHST- 2020/04/04 06:00 [medline]
PHST- 2019/06/18 06:00 [entrez]
AID - S0301-0546(19)30052-7 [pii]
AID - 10.1016/j.aller.2019.05.001 [doi]
PST - ppublish
SO  - Allergol Immunopathol (Madr). 2019 Nov-Dec;47(6):585-590. doi: 
      10.1016/j.aller.2019.05.001. Epub 2019 Jun 14.