PMID- 31207012
OWN - NLM
STAT- MEDLINE
DCOM- 20191119
LR  - 20191119
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Print)
IS  - 1526-498X (Linking)
VI  - 75
IP  - 9
DP  - 2019 Sep
TI  - Targeting the potassium ion channel genes SK and SH as a novel approach for 
      control of insect pests: efficacy and biosafety.
PG  - 2505-2516
LID - 10.1002/ps.5516 [doi]
AB  - BACKGROUND: Potassium ion channels play a critical role in the generation of 
      electrical signals and thus provide potential targets for control of insect pests 
      by RNA interference. RESULTS: Genes encoding the small conductance 
      calcium-activated potassium channel (SK) and the voltage-gated potassium channel 
      (SH) were knocked down in Tribolium castaneum by injection and oral delivery of 
      dsRNA (dsTcSK and dsTcSH, respectively). Irrespective of the delivery mechanism a 
      dose-dependent effect was observed for knockdown (KD) of gene expression and 
      insect mortality for both genes. Larvae fed a 400 ng dsRNA mg(-1) diet showed 
      significant gene (P < 0.05) knockdown (98% and 83%) for SK and SH, respectively, 
      with corresponding mortalities of 100% and 98% after 7 days. When injected 
      (248.4 ng larva(-1) ), gene KD was 99% and 98% for SK and SH, causing 100% and 
      73.4% mortality, respectively. All developmental stages tested (larvae, early- 
      and late-stage pupae and adults) showed an RNAi-sensitive response for both 
      genes. LC50 values were lower for SK than SH, irrespective of delivery method, 
      demonstrating that the knockdown of SK had a greater effect on larval mortality. 
      Biosafety studies using adult honeybee Apis mellifera showed that there were no 
      significant differences either in expression levels or mortality of honeybees 
      orally dosed with dsTcSK and dsTcSH compared to control-fed bees. Similarly, 
      there was no significant difference in the titre of deformed wing virus, used as 
      a measure of immune suppression, between experimental and control bees. 
      CONCLUSION: This study demonstrates the potential of using RNAi targeting neural 
      receptors as a technology for the control of T. castaneum. (c) 2019 The Authors. 
      Pest Management Science published by John Wiley & Sons Ltd on behalf of Society 
      of Chemical Industry.
CI  - (c) 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on 
      behalf of Society of Chemical Industry.
FAU - Alshukri, Baida
AU  - Alshukri B
AD  - School of Natural and Environmental Sciences, Newcastle University, 
      Newcastle-upon-Tyne, UK.
FAU - Astarita, Federica
AU  - Astarita F
AD  - School of Natural and Environmental Sciences, Newcastle University, 
      Newcastle-upon-Tyne, UK.
AD  - Department of Agricultural Sciences, Laboratory of Entomology "E. Tremblay", 
      University of Napoli "Federico II", Portici, Italy.
FAU - Al-Esawy, Mushtaq
AU  - Al-Esawy M
AD  - Institute of Neuroscience, Newcastle University, Newcastle-upon-Tyne, UK.
AD  - Department of Plant Protection, University of Kufa, Iraq.
FAU - El Halim, Hesham Mohamed El Sayed Abd
AU  - El Halim HMESA
AD  - School of Natural and Environmental Sciences, Newcastle University, 
      Newcastle-upon-Tyne, UK.
AD  - Entomology Department, Faculty of Science, Benha University, Benha, Egypt.
FAU - Pennacchio, Francesco
AU  - Pennacchio F
AD  - Department of Agricultural Sciences, Laboratory of Entomology "E. Tremblay", 
      University of Napoli "Federico II", Portici, Italy.
FAU - Gatehouse, Angharad Margaret Roscoe
AU  - Gatehouse AMR
AD  - School of Natural and Environmental Sciences, Newcastle University, 
      Newcastle-upon-Tyne, UK.
FAU - Edwards, Martin Gethin
AU  - Edwards MG
AUID- ORCID: 0000-0002-4863-653X
AD  - School of Natural and Environmental Sciences, Newcastle University, 
      Newcastle-upon-Tyne, UK.
LA  - eng
GR  - 773554/European Union's Horizon 2020 Research and Innovation programme/
GR  - BB/R022704/1/The Biotechnology and Biological Sciences Research Council/
PT  - Journal Article
DEP - 20190729
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
RN  - 0 (Insect Proteins)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (Small-Conductance Calcium-Activated Potassium Channels)
SB  - IM
MH  - Animals
MH  - Gene Knockdown Techniques
MH  - Insect Control/*methods
MH  - Insect Proteins/*antagonists & inhibitors
MH  - Larva/drug effects/genetics/growth & development
MH  - Potassium Channel Blockers/*metabolism
MH  - Pupa/drug effects/genetics/growth & development
MH  - RNA Interference
MH  - RNA, Double-Stranded/genetics
MH  - Small-Conductance Calcium-Activated Potassium Channels/antagonists & inhibitors
MH  - Tribolium/*drug effects/genetics/growth & development
PMC - PMC6771844
OTO - NOTNLM
OT  - Apis mellifera
OT  - RNAi
OT  - Tribolium castaneum
OT  - biosafety
OT  - insect control
OT  - potassium ion channels
EDAT- 2019/06/18 06:00
MHDA- 2019/11/20 06:00
PMCR- 2019/10/01
CRDT- 2019/06/18 06:00
PHST- 2018/11/30 00:00 [received]
PHST- 2019/06/04 00:00 [revised]
PHST- 2019/06/10 00:00 [accepted]
PHST- 2019/06/18 06:00 [pubmed]
PHST- 2019/11/20 06:00 [medline]
PHST- 2019/06/18 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - PS5516 [pii]
AID - 10.1002/ps.5516 [doi]
PST - ppublish
SO  - Pest Manag Sci. 2019 Sep;75(9):2505-2516. doi: 10.1002/ps.5516. Epub 2019 Jul 29.