PMID- 3120737
OWN - NLM
STAT- MEDLINE
DCOM- 19880121
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 36
IP  - 24
DP  - 1987 Dec 15
TI  - Ornithine decarboxylase activity in rabbit retina following treatment with 
      alpha-difluoromethylornithine.
PG  - 4325-9
AB  - The rabbit retina has been utilized as a model for the study of abnormal cellular 
      proliferation on the retinal surface and into the vitreous, a process commonly 
      initiated by trauma and generally leading to retinal detachment. This study 
      characterizes the ability of alpha-difluoromethylornithine (alpha-DFMO), a 
      suicide inactivator of L-ornithine decarboxylase (EC 4.1.1.17) to inactivate 
      normal retinal ornithine decarboxylase (ODC) activity in the crude supernatant 
      fraction after incubation with different concentrations of alpha-DFMO and at 
      various times after intraocular administration. Partial inactivation of ODC 
      activity occurred following preincubation of crude retinal supernatant fraction 
      with 10(-5) M alpha-DFMO (N = 3; 34 +/- 6.9% of control), whereas preincubation 
      with 10(-8) M alpha-DFMO did not alter ODC activity significantly (N = 3; 94 +/- 
      2% of control). Different concentrations of alpha-DFMO administered intraocularly 
      inactivated retinal ODC activity to varying degrees with different rates of 
      recovery. No gross toxicity occurred with ocular tissues following intravitreal 
      administration of alpha-DFMO as determined by electrophysiologic measurements, by 
      indirect examination of the retina, and by measurement of intraocular pressure. 
      These results suggest that alpha-DFMO may be a useful tool in which to define the 
      physiologic role of ODC and polyamines in intraocular cellular proliferative 
      diseases.
FAU - Wong, C G
AU  - Wong CG
AD  - Department of Ophthalmology, University of Southern California School of 
      Medicine, Los Angeles 90033.
FAU - Naripthaphan, P
AU  - Naripthaphan P
FAU - Renardel de Lavalette, V
AU  - Renardel de Lavalette V
LA  - eng
GR  - EYO-05810-061/EY/NEI NIH HHS/United States
GR  - EYO-4909-02/EY/NEI NIH HHS/United States
GR  - EYO-6147-01/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Ornithine Decarboxylase Inhibitors)
RN  - ZQN1G5V6SR (Eflornithine)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Eflornithine/administration & dosage/*pharmacology
MH  - In Vitro Techniques
MH  - *Ornithine Decarboxylase Inhibitors
MH  - Rabbits
MH  - Retina/*enzymology
MH  - Time Factors
EDAT- 1987/12/15 00:00
MHDA- 1987/12/15 00:01
CRDT- 1987/12/15 00:00
PHST- 1987/12/15 00:00 [pubmed]
PHST- 1987/12/15 00:01 [medline]
PHST- 1987/12/15 00:00 [entrez]
AID - 0006-2952(87)90679-4 [pii]
AID - 10.1016/0006-2952(87)90679-4 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1987 Dec 15;36(24):4325-9. doi: 10.1016/0006-2952(87)90679-4.