PMID- 31208517
OWN - NLM
STAT- MEDLINE
DCOM- 20190711
LR  - 20200808
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 21
IP  - 6
DP  - 2019 Jun
TI  - [Protective effect of early intervention with lipoxin A4 on septic mice].
PG  - 601-606
AB  - OBJECTIVE: To study the effect of early intervention with lipoxin A4 (LXA4) on 
      septic mice. METHODS: Healthy male Balb/c mice aged 6-8 weeks were randomly 
      divided into sham-operation group, sepsis group, 1-hour intervention group 
      (intervention at 1 hour after sepsis), and 6-hour intervention group 
      (intervention at 6 hours after sepsis) (n=8 each). A sepsis model was prepared by 
      cecal ligation and puncture. The intervention groups received LXA4 at 0.01 mug/g 
      body weight 1 or 6 hours after the model was established. Blood was taken from 
      eyeballs at 24 hours after operation. Peritoneal lavage fluid and liver and lung 
      tissue samples were collected. The bacterial colonies of whole blood and 
      peritoneal lavage fluid were counted by dilution plating. The serum levels of 
      tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and monocyte 
      chemoattractant protein-1 (MCP-1) were determined by cytometric bead array. The 
      serum level of high mobility group box-1 (HGMB1) was determined using ELISA. The 
      percentages of macrophages and neutrophils in peritoneal lavage fluid were 
      determined by flow cytometry. Paraffin sectioning and hematoxylin-eosin staining 
      were performed for the liver and lung tissue samples to observe pathological 
      damage. RESULTS: Compared with the sham-operation group, the sepsis group had a 
      significantly decreased percentage of macrophages and a significantly increased 
      percentage of neutrophils in peritoneal lavage fluid (P<0.05), as well as 
      significantly increased serum levels of IL-6, TNF-alpha, MCP-1, and HMGB1 (P<0.05); 
      in addition, the sepsis group showed more vacuolar degeneration, hepatocyte 
      swelling, and inflammatory cell infiltration in liver tissue, and more capillary 
      congestion, pulmonary septal thickening, inflammatory cell infiltration, and 
      partial tissue destruction in lung tissue. Compared with the sepsis group, the 
      1-hour and 6-hour intervention groups had a significantly increased percentage of 
      macrophages in peritoneal lavage fluid (P<0.05) and significantly reduced 
      bacterial load in whole blood (P<0.05), serum levels of IL-6, TNF-alpha, MCP-1, and 
      HMGB1 (P<0.05), and degree of liver and lung tissue damage and inflammatory cell 
      infiltration, but there was no significant difference in the percentage of 
      neutrophils and bacterial load in peritoneal lavage fluid (P>0.05). Compared with 
      the 6-hour intervention group, the 1-hour intervention group had a significantly 
      decreased serum level of HMGB1 (P<0.05), but there was no significant difference 
      in other indicators between the two groups (P>0.05). CONCLUSIONS: Early 
      intervention with LXA4 may attenuate liver and lung injuries in septic mice, 
      which may be explained by the decrease in serum levels of IL-6, TNF-alpha, MCP-1, and 
      HMGB1, and it also may reduce the bacterial dissemination in the whole blood of 
      septic mice, which may be explained by the increase in the percentage of 
      peritoneal macrophages.
FAU - Lin, Xing-Yun
AU  - Lin XY
AD  - The Generate School, Xuzhou Medical University, Xuzhou, Jiangsu 221000, China. 
      gaoll0808@sina.com.
FAU - Gao, Li-Li
AU  - Gao LL
FAU - Wu, Ming
AU  - Wu M
FAU - Zhao, Tong
AU  - Zhao T
FAU - Shen, Dong-Lin
AU  - Shen DL
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Interleukin-6)
RN  - 0 (Lipoxins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - 0 (lipoxin A4)
SB  - IM
MH  - Animals
MH  - Early Intervention, Educational
MH  - Interleukin-6
MH  - Lipoxins
MH  - Male
MH  - Mice
MH  - *Sepsis
MH  - Tumor Necrosis Factor-alpha
PMC - PMC7389568
EDAT- 2019/06/19 06:00
MHDA- 2019/07/12 06:00
PMCR- 2019/06/25
CRDT- 2019/06/19 06:00
PHST- 2019/06/19 06:00 [entrez]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2019/07/12 06:00 [medline]
PHST- 2019/06/25 00:00 [pmc-release]
AID - 10.7499/j.issn.1008-8830.2019.06.019 [pii]
AID - zgddekzz-21-6-601 [pii]
AID - 10.7499/j.issn.1008-8830.2019.06.019 [doi]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2019 Jun;21(6):601-606. doi: 
      10.7499/j.issn.1008-8830.2019.06.019.