PMID- 31208979
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200309
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 39
IP  - 17
DP  - 2019 Sep 1
TI  - Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells 
      and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.
LID - 10.1128/MCB.00154-19 [doi]
LID - e00154-19
AB  - The cancer/testis antigen (CTA) genes were identified as human genes 
      preferentially expressed in cancer cells and testis, but the contribution of CTAs 
      to cancer and male germ cell development is unclear. In this study, we 
      comprehensively examined mouse CTA functions and found that the majority of CTAs 
      are involved in growth and/or survival of cancer cells. We focused on one mouse 
      CTA gene, Tekt5, for its detailed functional analysis. Tekt5 knockdown (KD) in 
      ovarian cancer cells caused G(1) arrest and apoptosis, and p27(kip1) was 
      concomitantly upregulated. Tekt5 KD also resulted in decreased levels of 
      acetylated alpha-tubulin and subsequent fragmentation of beta-III-tubulin, upregulation 
      of HDAC6 that deacetylates alpha-tubulin, and nuclear accumulation of SMAD3 that 
      induces p27(kip1) expression. Because depolymerization of tubulin is known to 
      cause translocation of SMAD3 to the nucleus, these results together suggested 
      that TEKT5 negatively regulates Hdac6 expression and consequently maintains cell 
      cycle via stabilization of tubulin. We also found that the number of spermatids 
      was significantly decreased and acetylated alpha-tubulin levels were decreased in 
      vivo by KD of Tekt5 in testis. Because acetylated alpha-tubulin is required for sperm 
      morphogenesis, these results suggest that TEKT5 is necessary for spermiogenesis 
      via maintenance of acetylated alpha-tubulin levels.
CI  - Copyright (c) 2019 American Society for Microbiology.
FAU - Aoki, Nana
AU  - Aoki N
AD  - Cell Resource Center for Biomedical Research, Institute of Development, Aging and 
      Cancer, Tohoku University, Sendai, Miyagi, Japan.
AD  - Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.
FAU - Matsui, Yasuhisa
AU  - Matsui Y
AUID- ORCID: 0000-0001-7026-6355
AD  - Cell Resource Center for Biomedical Research, Institute of Development, Aging and 
      Cancer, Tohoku University, Sendai, Miyagi, Japan yasuhisa.matsui.d3@tohoku.ac.jp.
AD  - Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.
AD  - Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
AD  - The Japan Agency for Medical Research and Development-Core Research for 
      Evolutional Science and Technology (AMED-CREST), Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190812
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cdkn1b protein, mouse)
RN  - 0 (Microtubule Proteins)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (Tubulin)
RN  - 0 (tektins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - EC 3.5.1.98 (Hdac6 protein, mouse)
RN  - EC 3.5.1.98 (Histone Deacetylase 6)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cyclin-Dependent Kinase Inhibitor p27/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Histone Deacetylase 6/metabolism
MH  - Male
MH  - Mice
MH  - Microtubule Proteins/*genetics
MH  - Ovarian Neoplasms/*genetics
MH  - Protein Stability
MH  - Smad3 Protein/metabolism
MH  - *Spermatogenesis
MH  - Spermatozoa/*cytology/metabolism
MH  - Tubulin/chemistry/*metabolism
PMC - PMC6692122
OTO - NOTNLM
OT  - CTA
OT  - Tekt5
OT  - cancer cell
OT  - germ cell
EDAT- 2019/06/19 06:00
MHDA- 2020/01/28 06:00
PMCR- 2020/02/12
CRDT- 2019/06/19 06:00
PHST- 2019/04/10 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
PHST- 2019/06/19 06:00 [entrez]
PHST- 2020/02/12 00:00 [pmc-release]
AID - MCB.00154-19 [pii]
AID - 00154-19 [pii]
AID - 10.1128/MCB.00154-19 [doi]
PST - epublish
SO  - Mol Cell Biol. 2019 Aug 12;39(17):e00154-19. doi: 10.1128/MCB.00154-19. Print 
      2019 Sep 1.