PMID- 31209382
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20190718
IS  - 1548-7105 (Electronic)
IS  - 1548-7091 (Linking)
VI  - 16
IP  - 7
DP  - 2019 Jul
TI  - High-performance calcium sensors for imaging activity in neuronal populations and 
      microcompartments.
PG  - 649-657
LID - 10.1038/s41592-019-0435-6 [doi]
AB  - Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely 
      used to measure neural activity in intact nervous systems. GECIs are frequently 
      used in one of two different modes: to track activity in large populations of 
      neuronal cell bodies, or to follow dynamics in subcellular compartments such as 
      axons, dendrites and individual synaptic compartments. Despite major advances, 
      calcium imaging is still limited by the biophysical properties of existing GECIs, 
      including affinity, signal-to-noise ratio, rise and decay kinetics and dynamic 
      range. Using structure-guided mutagenesis and neuron-based screening, we 
      optimized the green fluorescent protein-based GECI GCaMP6 for different modes of 
      in vivo imaging. The resulting jGCaMP7 sensors provide improved detection of 
      individual spikes (jGCaMP7s,f), imaging in neurites and neuropil (jGCaMP7b), and 
      may allow tracking larger populations of neurons using two-photon (jGCaMP7s,f) or 
      wide-field (jGCaMP7c) imaging.
FAU - Dana, Hod
AU  - Dana H
AUID- ORCID: 0000-0002-8093-2363
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
AD  - Department of Neurosciences, Lerner Research Institute, Cleveland Clinic 
      Foundation, Cleveland, OH, USA.
FAU - Sun, Yi
AU  - Sun Y
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
AD  - School of Life Sciences, Westlake University, Hangzhou, China.
FAU - Mohar, Boaz
AU  - Mohar B
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Hulse, Brad K
AU  - Hulse BK
AUID- ORCID: 0000-0002-7117-7036
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Kerlin, Aaron M
AU  - Kerlin AM
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
AD  - Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
FAU - Hasseman, Jeremy P
AU  - Hasseman JP
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Tsegaye, Getahun
AU  - Tsegaye G
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Tsang, Arthur
AU  - Tsang A
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Wong, Allan
AU  - Wong A
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Patel, Ronak
AU  - Patel R
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Macklin, John J
AU  - Macklin JJ
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Chen, Yang
AU  - Chen Y
AD  - Institute of Neuroscience and SyNergy Cluster, Technical University of Munich, 
      Munich, Germany.
FAU - Konnerth, Arthur
AU  - Konnerth A
AD  - Institute of Neuroscience and SyNergy Cluster, Technical University of Munich, 
      Munich, Germany.
FAU - Jayaraman, Vivek
AU  - Jayaraman V
AUID- ORCID: 0000-0003-3680-7378
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. 
      jayaramanv@janelia.hhmi.org.
FAU - Looger, Loren L
AU  - Looger LL
AUID- ORCID: 0000-0002-7531-1757
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. 
      loogerl@janelia.hhmi.org.
FAU - Schreiter, Eric R
AU  - Schreiter ER
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Svoboda, Karel
AU  - Svoboda K
AUID- ORCID: 0000-0002-6670-7362
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. 
      svobodak@janelia.hhmi.org.
FAU - Kim, Douglas S
AU  - Kim DS
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
AD  - National Institute of Mental Health, Rockville, MD, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190617
PL  - United States
TA  - Nat Methods
JT  - Nature methods
JID - 101215604
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Drosophila
MH  - Female
MH  - Green Fluorescent Proteins
MH  - Mice
MH  - Neuromuscular Junction/diagnostic imaging
MH  - Neurons/*metabolism
MH  - Rats
MH  - Visual Cortex/metabolism
EDAT- 2019/06/19 06:00
MHDA- 2019/07/13 06:00
CRDT- 2019/06/19 06:00
PHST- 2018/10/03 00:00 [received]
PHST- 2019/04/30 00:00 [accepted]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
PHST- 2019/06/19 06:00 [entrez]
AID - 10.1038/s41592-019-0435-6 [pii]
AID - 10.1038/s41592-019-0435-6 [doi]
PST - ppublish
SO  - Nat Methods. 2019 Jul;16(7):649-657. doi: 10.1038/s41592-019-0435-6. Epub 2019 
      Jun 17.