PMID- 31210337
OWN - NLM
STAT- MEDLINE
DCOM- 20200925
LR  - 20210311
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 11
DP  - 2019 Jun
TI  - Chi3l1 regulates APAP-induced liver injury by promoting macrophage infiltration.
PG  - 4996-5003
LID - 18091 [pii]
LID - 10.26355/eurrev_201906_18091 [doi]
AB  - OBJECTIVE: This study aims to investigate the role of Chi3l1 in Acetaminophen 
      (APAP)-induced liver injury. MATERIALS AND METHODS: In vivo model of liver injury 
      was established in mice administrated with APAP (250 mg/kg) or equivalent 
      phosphate-buffered saline (PBS). Mouse liver tissues were collected at 1 h, 3 h, 
      6 h, 12 h, and 24 h after treatment, respectively. ALT levels and apoptosis were 
      evaluated. Additionally, we established APAP-induced acute liver injury model in 
      wild-type (WT) mice and Chi3l1-deficient (Chi3l1-/-) mice. Pathological changes 
      of liver tissue were observed by hematoxylin and eosin (HE) staining. Mononuclear 
      cells (MNCs) were isolated from mouse liver, and the amounts of infiltrating 
      macrophages and neutrophils were then counted by flow cytometry. Serum levels of 
      cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Bone 
      marrow-derived macrophages (BMDMs) were extracted from each mouse. RESULTS: After 
      APAP stimulation, Chi3l1-/- mice showed more severe liver injury than that of WT 
      mice, which was manifested as higher ALT levels and more necrotic or apoptotic 
      cells. Compared with WT mice, Chi3l1-/- mice expressed higher levels of 
      inflammatory cytokines (MCP-1 and IL-6), macrophage-associated molecules (CD68 
      and CD86), as well as the amounts of infiltrating macrophages and neutrophils. In 
      addition, higher expressions of inflammatory cytokines were found in BMDMs 
      extracted from WT mice treated with those BMDM lysates derived from Chi3l1-/- 
      mice than those of non-treated cells. APAP-treated Chi3l1-/- mice exhibited more 
      severe liver injury than that of WT mice. CONCLUSIONS: Our study confirmed that 
      Chi3l1 protects the liver function from APAP-induced injury by inhibiting the 
      secretion of inflammatory factors and macrophage infiltration.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of General Surgery, China-Japan Union Hospital of Jilin University, 
      Changchun, China. 43790964@qq.com.
FAU - Zhong, M
AU  - Zhong M
FAU - Wang, W
AU  - Wang W
FAU - Li, Y-H
AU  - Li YH
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Chil1 protein, mouse)
RN  - 0 (Chitinase-3-Like Protein 1)
RN  - 0 (Inflammation Mediators)
RN  - 362O9ITL9D (Acetaminophen)
SB  - IM
RIN - Eur Rev Med Pharmacol Sci. 2020 Oct;24(19):9775. PMID: 33090449
MH  - Acetaminophen/*toxicity
MH  - Animals
MH  - Cells, Cultured
MH  - Chemical and Drug Induced Liver Injury/diagnosis/*immunology/pathology
MH  - Chitinase-3-Like Protein 1/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Humans
MH  - Inflammation Mediators/immunology/metabolism
MH  - Liver/cytology/drug effects/*immunology/pathology
MH  - Macrophages/*immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Primary Cell Culture
EDAT- 2019/06/19 06:00
MHDA- 2020/09/26 06:00
CRDT- 2019/06/19 06:00
PHST- 2019/06/19 06:00 [entrez]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2020/09/26 06:00 [medline]
AID - 18091 [pii]
AID - 10.26355/eurrev_201906_18091 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Jun;23(11):4996-5003. doi: 
      10.26355/eurrev_201906_18091.