PMID- 31210429
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 21
IP  - 3
DP  - 2019 Oct
TI  - Preliminary Findings of Platelet-Rich Plasma-Induced Ameliorative Effect on 
      Polycystic Ovarian Syndrome.
PG  - 243-252
LID - 10.22074/cellj.2019.5952 [doi]
AB  - OBJECTIVE: Polycystic ovarian syndrome (PCOS) is characterized by hormonal 
      imbalance, oxidative stress and chronic anovulation. The present study was 
      designed to assess ameliorative effect of auto-locating platelet-rich plasma 
      (PRP), as a novel method, for inhibiting PCOS-induced pathogenesis in 
      experimentally-induced hyperandrogenic PCOS. MATERIALS AND METHODS: In this 
      experimental study, 30 immature (21 days old) female rats were assigned into five 
      groups, including control (sampled after 30 days with no treatment), 15 and 30 
      days PCOS-sole-induced as well as 15 and 30 days PRP auto-located PCOS-induced 
      groups. Serum levels of estrogen, progesterone, androstenedione, testosterone, 
      follicle stimulating hormone (FSH), luteinizing hormone (LH), ovarian total 
      antioxidant capacity (TAC), malondialdehyde (MDA), glutathione peroxidase 
      (GSH-px) and superoxide dismutase (SOD) were evaluated. Expression of estrogen 
      receptor alpha (Eralpha), beta (Erbeta) and c-Myc were assessed. Finally, the numbers of intact 
      follicles per ovary and mRNA damage ratio were analyzed. RESULTS: PRP groups 
      significantly (P<0.05) decreased serum levels of FSH, LH, testosterone and 
      androstenedione and remarkably (P<0.05) increased estrogen and progesterone 
      syntheses versus PCOS-sole groups. The PRP auto-located animals exhibited 
      increased TAC, GSH-px and SOD levels, while they showed diminished MDA content 
      (P<0.05) versus PCOS-sole groups. The PRP auto-located groups exhibited an 
      elevated expression of Eralpha and Erbeta versus PCOS-sole groups. Moreover, PRP groups 
      significantly (P<0.05) decreased c-Myc expression and mRNA damage compared to 
      PCOS-sole groups, and remarkably improved follicular growth. CONCLUSION: PRP is 
      able to regulate hormonal interaction, improve the ovarian antioxidant potential 
      as well as folliculogenesis and its auto-location could be considered as a novel 
      method to prevent/ameliorate PCOS-induced pathogenesis.
CI  - Copyright(c) by Royan Institute. All rights reserved.
FAU - Seyyed Anvari, Samira
AU  - Seyyed Anvari S
AD  - Department of Biology, Collage of Post Graduate, Ahar Islamic Azad University, 
      Ahar, Iran.
FAU - Dehgan, G Holamreza
AU  - Dehgan GH
AD  - Department of Biochemistry, Faculty of Natural Science, University of Tabriz, 
      Tabriz, Iran.
FAU - Razi, Mazdak
AU  - Razi M
AD  - Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, 
      Urmia, Iran.Electronic Address:mazdak.razi@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190615
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC6582424
OTO - NOTNLM
OT  - Folliculogenesis
OT  - Oxidative Stress
OT  - Platelet-Rich Plasma
OT  - Polycystic Ovarian Syndrome
OT  - Rat
COIS- There is no conflict of interest in this study.
EDAT- 2019/06/19 06:00
MHDA- 2019/06/19 06:01
PMCR- 2019/09/01
CRDT- 2019/06/19 06:00
PHST- 2018/04/04 00:00 [received]
PHST- 2018/11/19 00:00 [accepted]
PHST- 2019/06/19 06:00 [entrez]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2019/06/19 06:01 [medline]
PHST- 2019/09/01 00:00 [pmc-release]
AID - 10.22074/cellj.2019.5952 [doi]
PST - ppublish
SO  - Cell J. 2019 Oct;21(3):243-252. doi: 10.22074/cellj.2019.5952. Epub 2019 Jun 15.