PMID- 31211356
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20240214
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 160
IP  - 8
DP  - 2019 Aug 1
TI  - Menin Associates With the Mitotic Spindle and Is Important for Cell Division.
PG  - 1926-1936
LID - 10.1210/en.2019-00274 [doi]
AB  - Menin is the protein mutated in patients with multiple endocrine neoplasia type 1 
      (MEN1) syndrome and their corresponding sporadic tumor counterparts. We have 
      found that menin functions in promoting proper cell division. Here, we show that 
      menin localizes to the mitotic spindle poles and the mitotic spindle during early 
      mitosis and to the intercellular bridge microtubules during cytokinesis in HeLa 
      cells. In our study, menin depletion led to defects in spindle assembly and 
      chromosome congression during early mitosis, lagging chromosomes during anaphase, 
      defective cytokinesis, multinucleated interphase cells, and cell death. In 
      addition, pharmacological inhibition of the menin-MLL1 interaction also led to 
      similar cell division defects. These results indicate that menin and the 
      menin-MLL1 interaction are important for proper cell division. These results 
      highlight a function for menin in cell division and aid our understanding of how 
      mutation and misregulation of menin promotes tumorigenesis.
CI  - Copyright (c) 2019 Endocrine Society.
FAU - Sawicki, Mark P
AU  - Sawicki MP
AD  - Department of Surgery, VA Greater Los Angeles Healthcare System, Los Angeles, 
      California.
AD  - Department of Surgery, University of California, Los Angeles David Geffen School 
      of Medicine, Los Angeles, California.
AD  - Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 
      California.
FAU - Gholkar, Ankur A
AU  - Gholkar AA
AD  - Department of Chemistry and Biochemistry, University of California, Los Angeles, 
      California.
FAU - Torres, Jorge Z
AU  - Torres JZ
AD  - Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 
      California.
AD  - Department of Chemistry and Biochemistry, University of California, Los Angeles, 
      California.
AD  - Molecular Biology Institute, University of California, Los Angeles, California.
LA  - eng
GR  - T32 GM007185/GM/NIGMS NIH HHS/United States
GR  - UL1 TR001881/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (KMT2A protein, human)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - *Cell Division
MH  - HCT116 Cells
MH  - HeLa Cells
MH  - Histone-Lysine N-Methyltransferase/physiology
MH  - Humans
MH  - Myeloid-Lymphoid Leukemia Protein/physiology
MH  - Proto-Oncogene Proteins/genetics/*physiology
MH  - Spindle Apparatus/*physiology
PMC - PMC6656424
EDAT- 2019/06/19 06:00
MHDA- 2019/12/18 06:00
PMCR- 2020/06/18
CRDT- 2019/06/19 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2019/06/11 00:00 [accepted]
PHST- 2019/06/19 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/06/19 06:00 [entrez]
PHST- 2020/06/18 00:00 [pmc-release]
AID - 5519300 [pii]
AID - 201900274 [pii]
AID - 10.1210/en.2019-00274 [doi]
PST - ppublish
SO  - Endocrinology. 2019 Aug 1;160(8):1926-1936. doi: 10.1210/en.2019-00274.