PMID- 31212992
OWN - NLM
STAT- MEDLINE
DCOM- 20200107
LR  - 20200225
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 11
IP  - 6
DP  - 2019 Jun 17
TI  - The Role of Physiological Vitamin C Concentrations on Key Functions of 
      Neutrophils Isolated from Healthy Individuals.
LID - 10.3390/nu11061363 [doi]
LID - 1363
AB  - Vitamin C (ascorbate) is important for neutrophil function and immune health. 
      Studies showing improved immune function have primarily used cells from scorbutic 
      animals or from individuals with infectious conditions or immune cell disorders. 
      Few studies have focused on the requirements of neutrophils from healthy adults. 
      Therefore, we have investigated the role of vitamin C, at concentrations 
      equivalent to those obtained in plasma from oral intakes (i.e., 50-200 micromol/L), 
      on key functions of neutrophils isolated from healthy individuals. Cells were 
      either pre-loaded with dehydroascorbic acid, which is rapidly reduced 
      intracellularly to ascorbate, or the cells were activated in the presence of 
      extracellular ascorbate. We measured the effects of enhanced ascorbate uptake on 
      the essential functions of chemotaxis, oxidant production, programmed cell death 
      and neutrophil extracellular trap (NET) formation. We found that neutrophils 
      isolated from healthy individuals already had replete ascorbate status (0.35 
      nmol/10(6) cells), therefore they did not uptake additional ascorbate. However, 
      they readily took up dehydroascorbic acid, thus significantly increasing their 
      intracellular ascorbate concentrations, although this was found to have no 
      additional effect on superoxide production or chemotaxis. Interestingly, 
      extracellular ascorbate appeared to enhance directional mobilityin the presence 
      of the chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP). Stimulation 
      of the cells in the presence of ascorbate significantly increased intracellular 
      ascorbate concentrations and, although this exhibited a non-significant increase 
      in phosphatidylserine exposure, NET formation was significantly attenuated. Our 
      findings demonstrate the ability of neutrophils to regulate their uptake of 
      ascorbate from the plasma of healthy humans to maintain an optimal level within 
      the cell for proper functioning. Higher oral intakes, however, may help reduce 
      tissue damage and inflammatory pathologies associated with NET formation.
FAU - Bozonet, Stephanie M
AU  - Bozonet SM
AD  - Department of Pathology and Biomedical Science, University of Otago, 
      Christchurch, P.O. Box 4345, Christchurch 8140, New Zealand. 
      stephanie.bozonet@otago.ac.nz.
FAU - Carr, Anitra C
AU  - Carr AC
AUID- ORCID: 0000-0002-5890-2977
AD  - Department of Pathology and Biomedical Science, University of Otago, 
      Christchurch, P.O. Box 4345, Christchurch 8140, New Zealand. 
      anitra.carr@otago.ac.nz.
LA  - eng
GR  - NA/Bayer Consumer Care/
PT  - Journal Article
DEP - 20190617
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - Y2Z3ZTP9UM (Dehydroascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/*physiology
MH  - Biological Transport
MH  - Chemotaxis
MH  - Dehydroascorbic Acid/metabolism
MH  - Extracellular Traps/metabolism
MH  - Healthy Volunteers
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism
MH  - Neutrophils/*metabolism
PMC - PMC6627200
OTO - NOTNLM
OT  - ascorbate
OT  - chemotaxis
OT  - immunity
OT  - neutrophil extracellular traps
OT  - neutrophils
OT  - vitamin C
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2019/06/20 06:00
MHDA- 2020/01/08 06:00
PMCR- 2019/06/01
CRDT- 2019/06/20 06:00
PHST- 2019/05/09 00:00 [received]
PHST- 2019/05/28 00:00 [revised]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/20 06:00 [entrez]
PHST- 2019/06/20 06:00 [pubmed]
PHST- 2020/01/08 06:00 [medline]
PHST- 2019/06/01 00:00 [pmc-release]
AID - nu11061363 [pii]
AID - nutrients-11-01363 [pii]
AID - 10.3390/nu11061363 [doi]
PST - epublish
SO  - Nutrients. 2019 Jun 17;11(6):1363. doi: 10.3390/nu11061363.