PMID- 31213128
OWN - NLM
STAT- MEDLINE
DCOM- 20200320
LR  - 20200619
IS  - 1555-8584 (Electronic)
IS  - 1547-6286 (Print)
IS  - 1547-6286 (Linking)
VI  - 16
IP  - 9
DP  - 2019 Sep
TI  - Circular RNA profile of parathyroid neoplasms: analysis of co-expression networks 
      of circular RNAs and mRNAs.
PG  - 1228-1236
LID - 10.1080/15476286.2019.1622962 [doi]
AB  - Circular RNAs (circRNAs) are a recently identified class of non-coding RNAs that 
      participate in multiple biological processes and tumour progression. However, 
      circRNA expression pattern in parathyroid neoplasms remains unknown. The circRNA 
      profile of 6 parathyroid carcinomas (PCs), 6 parathyroid adenomas (PAs) and 4 
      normal parathyroid tissues was assessed by a microarray. Bioinformatic analyses 
      were performed to investigate potential core circRNAs via co-expression network. 
      CircRNA and corresponding mRNA expression were validated in a cohort of 
      parathyroid neoplasms by RT-qPCR and fluorescence in situ hybridization (FISH). 
      Compared to normal parathyroid, 5310 and 1055 circRNAs were differentially 
      expressed in PC and PA tissues, respectively. The differential expression of 4 
      circRNAs (hsa_circRNA_0035563 (p = 0.006), hsa_circRNA_0017545 (p = 0.009), 
      hsa_circRNA_0001687 (p = 0.005) and hsa_circRNA_0075005 (p = 0.001)) and 4 mRNAs 
      (MYC, FSCN1, ANXA2 and AKR1C3) between PC and PA tissues were confirmed by 
      RT-qPCR. In addition, high expression of hsa_circ_0035563 was related to CDC73 
      mutations (p = 0.022) and recurrence in PC patients (p = 0.042). Furthermore, 
      hsa_circ_0075005 helped distinguish PCs from benign lesions using FISH, and the 
      area under the curve was 0.779 (p = 0.013). Our findings describe the circRNA 
      profile of PC for the first time and suggest that circRNAs and mRNAs interact in 
      parathyroid tumourigenesis. This study demonstrates that hsa_circ_0075005 and MYC 
      mRNA may be used for the differential diagnosis of PC and PA. The expression 
      levels of hsa_circ_0035563 are related to CDC73 mutations and recurrence in 
      malignancy, highlighting the significance of this parameter in prognosis of PC 
      patients.
FAU - Hu, Ya
AU  - Hu Y
AUID- ORCID: 0000-0003-2813-9947
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Zhang, Xiang
AU  - Zhang X
AUID- ORCID: 0000-0001-6047-0934
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Cui, Ming
AU  - Cui M
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Wang, Mengyi
AU  - Wang M
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Su, Zhe
AU  - Su Z
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Liao, Quan
AU  - Liao Q
AUID- ORCID: 0000-0003-1324-6213
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
FAU - Zhao, Yupei
AU  - Zhao Y
AD  - a Department of General Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College , Beijing , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190618
PL  - United States
TA  - RNA Biol
JT  - RNA biology
JID - 101235328
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Parathyroid Glands/metabolism
MH  - Parathyroid Neoplasms/*genetics/pathology
MH  - RNA, Circular/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reproducibility of Results
MH  - Statistics, Nonparametric
PMC - PMC6693544
OTO - NOTNLM
OT  - CDC73
OT  - Parathyroid carcinoma
OT  - circular RNA
OT  - co-expression network
OT  - mRNA
EDAT- 2019/06/20 06:00
MHDA- 2020/03/21 06:00
PMCR- 2020/06/18
CRDT- 2019/06/20 06:00
PHST- 2019/06/20 06:00 [pubmed]
PHST- 2020/03/21 06:00 [medline]
PHST- 2019/06/20 06:00 [entrez]
PHST- 2020/06/18 00:00 [pmc-release]
AID - 1622962 [pii]
AID - 10.1080/15476286.2019.1622962 [doi]
PST - ppublish
SO  - RNA Biol. 2019 Sep;16(9):1228-1236. doi: 10.1080/15476286.2019.1622962. Epub 2019 
      Jun 18.