PMID- 31214288
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1976-1457 (Print)
IS  - 2005-6168 (Electronic)
IS  - 1976-1457 (Linking)
VI  - 13
IP  - 3
DP  - 2019 Jun
TI  - Supplementation with psyllium seed husk reduces myocardial damage in a rat model 
      of ischemia/reperfusion.
PG  - 205-213
LID - 10.4162/nrp.2019.13.3.205 [doi]
AB  - BACKGROUND/OBJECTIVES: Myocardial infarction (MI) is caused by extensive 
      myocardial damage attributed to the occlusion of coronary arteries. Our previous 
      study in a rat model of ischemia/reperfusion (I/R) demonstrated that 
      administration of arabinoxylan (AX), comprising arabinose and xylose, protects 
      against myocardial injury. In this study, we undertook to investigate whether 
      psyllium seed husk (PSH), a safe dietary fiber containing a high level of AX (> 
      50%), also imparts protection against myocardial injury in the same rat model. 
      MATERIALS/METHODS: Rats were fed diets supplemented with PSH (1, 10, or 100 
      mg/kg/d) for 3 d. The rats were then subjected to 30 min ischemia through 
      ligation of the left anterior descending coronary artery, followed by 3 h 
      reperfusion through release of the ligation. The hearts were harvested and cut 
      into four slices. To assess infarct size (IS), an index representing heart 
      damage, the slices were stained with 2,3,5-triphenyltetrazolium chloride (TTC). 
      To elucidate underlying mechanisms, Western blotting was performed for the 
      slices. RESULTS: Supplementation with 10 or 100 mg/kg/d of PSH significantly 
      reduces the IS. PSH supplementation (100 mg/kg/d) tends to reduce caspase-3 
      generation and increase BCL-2/BAX ratio. PSH supplementation also upregulates the 
      expression of nuclear factor erythroid 2-related factor 2 (NRF2), and its target 
      genes including antioxidant enzymes such as glutathione S-transferase mu 2 
      (GSTM2) and superoxide dismutase 2 (SOD2). PSH supplementation upregulates some 
      sirtuins (NAD(+)-dependent deacetylases) including SIRT5 (a mitochondrial 
      sirtuin) and SIRT6 and SIRT7 (nuclear sirtuins). Finally, PSH supplementation 
      upregulates the expression of protein kinase A (PKA), and increases 
      phosphorylated cAMP response element-binding protein (CREB) (pCREB), a target 
      protein of PKA. CONCLUSIONS: The results from this study indicate that PSH 
      consumption reduces myocardial I/R injury in rats by inhibiting the apoptotic 
      cascades through modulation of gene expression of several genes located upstream 
      of apoptosis. Therefore, we believe that PSH can be developed as a functional 
      food that would be beneficial in the prevention of MI.
FAU - Lim, Sun Ha
AU  - Lim SH
AUID- ORCID: 0000-0002-8371-4740
AD  - Department of Biochemistry, School of Medicine, Catholic University of Daegu, 33 
      Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of Korea.
FAU - Lee, Jongwon
AU  - Lee J
AUID- ORCID: 0000-0001-9372-9850
AD  - Department of Biochemistry, School of Medicine, Catholic University of Daegu, 33 
      Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20190507
PL  - Korea (South)
TA  - Nutr Res Pract
JT  - Nutrition research and practice
JID - 101311052
PMC - PMC6548711
OTO - NOTNLM
OT  - Infarction
OT  - apoptosis
OT  - functional food
OT  - sirtuin
COIS- CONFLICT OF INTEREST: The authors declare no potential conflicts of interests.
EDAT- 2019/06/20 06:00
MHDA- 2019/06/20 06:01
PMCR- 2019/06/01
CRDT- 2019/06/20 06:00
PHST- 2018/12/17 00:00 [received]
PHST- 2019/03/13 00:00 [revised]
PHST- 2019/03/23 00:00 [accepted]
PHST- 2019/06/20 06:00 [entrez]
PHST- 2019/06/20 06:00 [pubmed]
PHST- 2019/06/20 06:01 [medline]
PHST- 2019/06/01 00:00 [pmc-release]
AID - 10.4162/nrp.2019.13.3.205 [doi]
PST - ppublish
SO  - Nutr Res Pract. 2019 Jun;13(3):205-213. doi: 10.4162/nrp.2019.13.3.205. Epub 2019 
      May 7.