PMID- 31215398
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 20
IP  - 1
DP  - 2019 Jun 18
TI  - Comprehensive transcriptomic view of the role of the LGALS12 gene in porcine 
      subcutaneous and intramuscular adipocytes.
PG  - 509
LID - 10.1186/s12864-019-5891-y [doi]
LID - 509
AB  - BACKGROUND: Livestock production aims to provide meats of high and consistent 
      eating quality. Insufficient intramuscular (IM) fat and excessive subcutaneous 
      (SC) fat are paramount pork quality challenges. IM fat and SC fat, which are 
      modulated by the adipogenesis of IM and SC adipocytes, play key roles in pork 
      quality. Galectin-12 (LGALS12) was proven to be an important regulator of fat 
      deposition in porcine. However, the current knowledge of the transcriptome-wide 
      role of LGALS12 in adipocytes is still limited. This study was aimed to discover 
      the different regulatory mechanisms of LGALS12 in porcine IM and SC adipocyte. 
      RESULTS: The siRNA-mediated knockdown of the expression of LGALS12 identified 
      1075 and 3016 differentially expressed genes (DEGs) in IM and SC adipocytes, 
      respectively. Among these, 585 were up- and 490 were downregulated in the IM 
      adipocytes, while 2186 were up- and 830 were downregulated in the SC adipocytes. 
      Moreover, 418 DGEs were observed only in the IM adipocytes, 2359 DGEs only in the 
      SC adipocytes, and 657 DGEs in both types of adipocytes. According to Gene 
      Ontology (GO) analysis, DEGs in both IM and SC adipocytes were mainly enriched in 
      categories related to lipids or fat cell differentiation. Pathway analysis of the 
      DEGs revealed 88 changed signaling pathways in the IM adipocytes and 86 in the SC 
      adipocytes. The signaling pathways present in only one type of adipocyte were 
      identified from among the top 50 signaling pathways in each type of adipocyte. 
      Four signaling pathways, encompassing PI3K-AKT, cardiac muscle contraction, fatty 
      acid metabolism and Ras, were significantly enriched in the IM adipocytes. On the 
      other hand, four different signaling pathways, encompassing TNF, WNT, cGMP-PKG 
      and NF-kappa B, were greatly enriched in the SC ones. The pathway changes were 
      confirmed by chemical inhibition assays. CONCLUSIONS: Our data reveals that 
      LGALS12 knockdown alters the expression of numerous genes involved in key 
      biological processes in the development of adipocytes. These observations provide 
      a global view of the role of LGALS12 in porcine IM and SC adipocytes; thus, 
      improving our understanding of the regulatory mechanisms by which this gene acts 
      in fat development.
FAU - Wu, Wenjing
AU  - Wu W
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing, 314001, China.
FAU - Zhang, Dawei
AU  - Zhang D
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing, 314001, China.
FAU - Yin, Yajun
AU  - Yin Y
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing, 314001, China.
FAU - Ji, Miao
AU  - Ji M
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qin Huangdao Hebei, 066000, China.
FAU - Xu, Ke
AU  - Xu K
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qin Huangdao Hebei, 066000, China.
FAU - Huang, Xin
AU  - Huang X
AD  - College of Agronomy and Biotechnology, Hebei Normal University of Science and 
      Technology, Qin Huangdao Hebei, 066000, China.
FAU - Peng, Yongjia
AU  - Peng Y
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing, 314001, China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - College of Biological, Chemical Sciences and Engineering, Jiaxing University, 
      Jiaxing, 314001, China. zhangjin7688@163.com.
LA  - eng
GR  - LQ18C170002/Zhejiang Natural Science Foundation/
GR  - LY17C170003/Zhejiang Natural Science Foundation/
GR  - C2014407083/Hebei Natural Science Foundation for Distinguished Young Scientists/
GR  - 70516055/Initial Scientific Research Fund of Young Teachers in Jiaxing 
      University/
GR  - Y201738243/General Research Project of Zhejiang Provincial Department of Education/
PT  - Journal Article
DEP - 20190618
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Galectins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Galectins/deficiency/*genetics
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/genetics
MH  - Gene Ontology
MH  - Gene Silencing
MH  - Muscles/*cytology
MH  - Subcutaneous Fat/*cytology
MH  - Swine/*metabolism
MH  - Transcription Factors/metabolism
PMC - PMC6582507
OTO - NOTNLM
OT  - Intramuscular adipocyte
OT  - LGALS12
OT  - RNA-seq
OT  - Subcutaneous adipocyte
OT  - Sus scrofa
COIS- The authors declare that they have no competing interests.
EDAT- 2019/06/20 06:00
MHDA- 2020/03/17 06:00
PMCR- 2019/06/18
CRDT- 2019/06/20 06:00
PHST- 2019/02/28 00:00 [received]
PHST- 2019/06/10 00:00 [accepted]
PHST- 2019/06/20 06:00 [entrez]
PHST- 2019/06/20 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2019/06/18 00:00 [pmc-release]
AID - 10.1186/s12864-019-5891-y [pii]
AID - 5891 [pii]
AID - 10.1186/s12864-019-5891-y [doi]
PST - epublish
SO  - BMC Genomics. 2019 Jun 18;20(1):509. doi: 10.1186/s12864-019-5891-y.