PMID- 31218457 OWN - NLM STAT- MEDLINE DCOM- 20200224 LR - 20200224 IS - 1573-6814 (Electronic) IS - 1389-9333 (Linking) VI - 20 IP - 3 DP - 2019 Sep TI - Extracellular matrix derived by human umbilical cord-deposited mesenchymal stem cells accelerates chondrocyte proliferation and differentiation potential in vitro. PG - 351-365 LID - 10.1007/s10561-019-09774-7 [doi] AB - The extracellular matrix (ECM) is a dynamic and intricate three-dimensional (3D) microenvironment with excellent biophysical, biomechanical, and biochemical properties that may directly or indirectly regulate cell behavior, including proliferation, adhesion, migration, and differentiation. Compared with tissue-derived ECM, cell-derived ECM potentially has more advantages, including less potential for pathogen transfer, fewer inflammatory or anti-host immune responses, and a closer resemblance to the native ECM microenvironment. Different types of cell-derived ECM, such as adipose stem cells, synovium-derived stem cells and bone marrow stromal cells, their effects on articular chondrocytes which have been researched. In this study, we aimed to develop a 3D cell culture substrate using decellularized ECM derived from human umbilical cord-derived mesenchymal stem cells (hUCMSCs), and evaluated the effects on articular chondrocytes. We evaluated the morphology and components of hUCMSC-derived ECM using physical and chemical methods. Morphological, histological, immunohistochemical, biochemical, and real-time PCR analyses demonstrated that proliferation and differentiation capacity of chondrocytes using the 3D hUCMSC-derived ECM culture substrate was superior to that using non-coated two-dimensional plastic culture plates. In conclusion, 3D decellularized ECM derived from hUCMSCs offers a tissue-specific microenvironment for in vitro culture of chondrocytes, which not only markedly promoted chondrocyte proliferation but also preserved the differentiation capacity of chondrocytes. Therefore, our findings suggest that a 3D cell-derived ECM microenvironment represents a promising prospect for autologous chondrocyte-based cartilage tissue engineering and regeneration. The hUCMSC-derived ECM as a biomaterial is used for the preparation of scaffold or hybrid scaffold products which need to further study in the future. FAU - Zhang, Weixiang AU - Zhang W AD - The People's Hospital of Lanxi, 1359th Shanxi Road, Lanxi, 321100, China. AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Yang, Jianhua AU - Yang J AD - School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. FAU - Zhu, Yun AU - Zhu Y AD - School of Biomedical Sciences, The University of Hong Kong, 21 Sassoon Road, Pokfulam, 999777, Hong Kong. FAU - Sun, Xun AU - Sun X AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. AD - School of Medicine, Nankai University, 94th Weijin Road, Nankai District, Tianjin, 300071, China. FAU - Guo, Weimin AU - Guo W AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Liu, Xuejian AU - Liu X AD - School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. AD - Zhengzhou Yihe Hospital Affiliated to Henan University, 69th Nongyedong Road, Zhengzhou, 450000, China. FAU - Jing, Xiaoguang AU - Jing X AD - School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Guo, Ganggang AU - Guo G AD - School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Guo, Quanyi AU - Guo Q AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Peng, Jiang AU - Peng J AD - Institute of Orthopedics, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, China. FAU - Zhu, Xiaofeng AU - Zhu X AD - School of Medicine, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. zhuxiaofeng1227@163.com. AD - Medical Research Center of Mudanjiang Medical School, 3th Tongxiang Road, Aimin District, Mudanjiang, 157011, China. zhuxiaofeng1227@163.com. AD - Institute of Neurosciences, Jiamusi University, 148th Xuefu Road, Xiangyang District, Jiamusi, 154007, China. zhuxiaofeng1227@163.com. LA - eng GR - 2014CB542201/the National Basic Research Program of China/ PT - Journal Article DEP - 20190619 PL - Netherlands TA - Cell Tissue Bank JT - Cell and tissue banking JID - 100965121 RN - 0 (Biocompatible Materials) RN - 0 (Fibronectins) SB - IM MH - Adipose Tissue/cytology MH - Animals MH - Biocompatible Materials/metabolism MH - Cartilage, Articular/cytology MH - Cell Adhesion MH - Cell Culture Techniques MH - *Cell Differentiation MH - Cell Movement MH - *Cell Proliferation MH - Chondrocytes/*cytology/metabolism MH - Chondrogenesis MH - Extracellular Matrix/*metabolism MH - Fibronectins/chemistry MH - Humans MH - Inflammation MH - Mesenchymal Stem Cells/*cytology MH - Rabbits MH - Tissue Engineering/instrumentation MH - Tissue Scaffolds MH - Umbilical Cord/*cytology OTO - NOTNLM OT - 3D microenvironment OT - Cell-derived ECM OT - Chondrocytes OT - Proliferation OT - Stem cells EDAT- 2019/06/21 06:00 MHDA- 2020/02/25 06:00 CRDT- 2019/06/21 06:00 PHST- 2019/01/17 00:00 [received] PHST- 2019/05/10 00:00 [accepted] PHST- 2019/06/21 06:00 [pubmed] PHST- 2020/02/25 06:00 [medline] PHST- 2019/06/21 06:00 [entrez] AID - 10.1007/s10561-019-09774-7 [pii] AID - 10.1007/s10561-019-09774-7 [doi] PST - ppublish SO - Cell Tissue Bank. 2019 Sep;20(3):351-365. doi: 10.1007/s10561-019-09774-7. Epub 2019 Jun 19.