PMID- 31218550
OWN - NLM
STAT- MEDLINE
DCOM- 20200323
LR  - 20210311
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 74
IP  - 1
DP  - 2020 Jan
TI  - Naringenin suppresses neutrophil infiltration into adipose tissue in high-fat 
      diet-induced obese mice.
PG  - 229-237
LID - 10.1007/s11418-019-01332-5 [doi]
AB  - Recruitment of immune cells to adipose tissue is altered dramatically in obesity, 
      which results in chronic inflammation of the adipose tissue that leads to 
      metabolic disorders, such as insulin resistance and type 2 diabetes mellitus. The 
      regulation of immune cell infiltration into adipose tissue has prophylactic and 
      therapeutic implications for obesity-related diseases. We previously showed that 
      naringenin, a citrus flavonoid, suppressed macrophage infiltration into adipose 
      tissue by inhibiting monocyte chemoattractant protein-1 (MCP-1) expression in the 
      progression phase to high-fat diet (HFD)-induced obesity. In the current study, 
      we evaluated the effects of naringenin on neutrophil infiltration into adipose 
      tissue, because neutrophils also infiltrate into adipose tissue in the 
      progression phase to obesity. Naringenin suppressed neutrophil infiltration into 
      adipose tissue induced by the short-term (2 weeks) feeding of a HFD to mice. 
      Naringenin tended to inhibit the HFD-induced expression of several chemokines, 
      including MCP-1 and MCP-3, in adipose tissue. Naringenin also inhibited MCP-3 
      expression in 3T3-L1 adipocytes and a co-culture of 3T3-L1 adipocytes and RAW264 
      macrophages. However, naringenin did not affect the expression of macrophage 
      inflammatory protein-2 (MIP-2), an important chemokine for neutrophil migration 
      and activation, in macrophages or in a co-culture of adipocytes and macrophages. 
      Our results suggest that naringenin suppresses neutrophil infiltration into 
      adipose tissue via the regulation of MCP-3 expression and macrophage 
      infiltration.
FAU - Tsuhako, Rika
AU  - Tsuhako R
AD  - Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu 
      University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, 882-8508, 
      Japan.
FAU - Yoshida, Hiroki
AU  - Yoshida H
AUID- ORCID: 0000-0002-6903-2401
AD  - Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu 
      University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, 882-8508, 
      Japan. h-yoshida@phoenix.ac.jp.
FAU - Sugita, Chihiro
AU  - Sugita C
AD  - Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu 
      University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, 882-8508, 
      Japan.
FAU - Kurokawa, Masahiko
AU  - Kurokawa M
AD  - Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu 
      University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, 882-8508, 
      Japan.
LA  - eng
GR  - JP15K18949/Grant-in-Aid for Scientific Research/
GR  - N-177401/Nagai Memorial Research Scholarship/
GR  - H30-06/Research Grant from Kyushu University of Health and Welfare/
PT  - Journal Article
DEP - 20190619
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccl7 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL7)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Cxcl2 protein, mouse)
RN  - 0 (Flavanones)
RN  - HN5425SBF2 (naringenin)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/metabolism
MH  - Adipose Tissue/*cytology/metabolism
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemokine CCL7/*biosynthesis
MH  - Chemokine CXCL2/*biosynthesis
MH  - Coculture Techniques
MH  - Diabetes Mellitus, Type 2/pathology
MH  - Diet, High-Fat
MH  - Flavanones/*pharmacology
MH  - Inflammation/pathology
MH  - Insulin Resistance/physiology
MH  - Macrophages/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Neutrophil Infiltration/*drug effects
MH  - Obesity/pathology
MH  - RAW 264.7 Cells
OTO - NOTNLM
OT  - MCP-3
OT  - MIP-2
OT  - Macrophage
OT  - Naringenin
OT  - Neutrophil
OT  - Obesity
EDAT- 2019/06/21 06:00
MHDA- 2020/03/24 06:00
CRDT- 2019/06/21 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/06/11 00:00 [accepted]
PHST- 2019/06/21 06:00 [pubmed]
PHST- 2020/03/24 06:00 [medline]
PHST- 2019/06/21 06:00 [entrez]
AID - 10.1007/s11418-019-01332-5 [pii]
AID - 10.1007/s11418-019-01332-5 [doi]
PST - ppublish
SO  - J Nat Med. 2020 Jan;74(1):229-237. doi: 10.1007/s11418-019-01332-5. Epub 2019 Jun 
      19.