PMID- 31218890
OWN - NLM
STAT- MEDLINE
DCOM- 20191202
LR  - 20191202
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 47
IP  - 1
DP  - 2019 Dec
TI  - Vacuolar protein sorting 4B regulates the proliferation and odontoblastic 
      differentiation of human dental pulp stem cells through the Wnt-beta-catenin 
      signalling pathway.
PG  - 2575-2584
LID - 10.1080/21691401.2019.1629950 [doi]
AB  - Our previous studies have revealed that a dominant mutation in vacuolar protein 
      sorting 4B (VPS4B), a member of the AAA ATPase family, causes dentin dysplasia 
      type I. The purpose of the present study was to investigate the roles of VPS4B in 
      human dental pulp stem cells (hDPSCs) and to elucidate the underlying molecular 
      mechanisms. In this study, we found that VPS4B was highly expressed in the dental 
      pulp cells of the mouse molar tooth germ, and the expression of VPS4B increased 
      significantly during the odontoblastic differentiation of hDPSCs. VPS4B 
      downregulation inhibited the proliferation, migration, and odontoblastic 
      differentiation of hDPSCs. Moreover, treatment with lithium chloride, an agonist 
      of the Wnt-beta-catenin signalling pathway, partially reversed the VPS4B 
      knockdown-driven suppression of proliferation and of odontoblastic 
      differentiation of hDPSCs. Collectively, our findings indicate that VPS4B, via 
      Wnt-beta-catenin signalling, acts as a regulator of the proliferation and 
      differentiation of hDPSCs. Our results suggest potential therapeutic avenues for 
      dentin formation and regenerative endodontics in patients with dentin dysplasia 
      type I.
FAU - Pan, Yuhua
AU  - Pan Y
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
FAU - Lu, Ting
AU  - Lu T
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
FAU - Peng, Ling
AU  - Peng L
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
FAU - Chen, Zhao
AU  - Chen Z
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
FAU - Li, Meiyi
AU  - Li M
AD  - b Department of Medical Genetics, School of Basic Medicine Sciences, Southern 
      Medical University , Guangzhou , China.
AD  - c Guangdong Provincial Key Laboratory of Single-Cell Technology and Application , 
      Guangzhou , China.
FAU - Zhang, Kaiying
AU  - Zhang K
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
FAU - Xiong, Fu
AU  - Xiong F
AD  - b Department of Medical Genetics, School of Basic Medicine Sciences, Southern 
      Medical University , Guangzhou , China.
AD  - c Guangdong Provincial Key Laboratory of Single-Cell Technology and Application , 
      Guangzhou , China.
FAU - Wu, Buling
AU  - Wu B
AD  - a Department of Stomatology, Nanfang Hospital, Southern Medical University , 
      Guangzhou , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta Catenin)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (VPS4B protein, human)
RN  - EC 3.6.4.6 (Vps4b protein, mouse)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities/genetics/*metabolism
MH  - Animals
MH  - *Cell Differentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Dental Pulp/*cytology
MH  - Down-Regulation
MH  - Endosomal Sorting Complexes Required for Transport/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Odontoblasts/*cytology
MH  - Protein Transport
MH  - RNA, Messenger/genetics/metabolism
MH  - Stem Cells/*cytology
MH  - *Wnt Signaling Pathway
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - Vacuolar protein sorting 4B
OT  - Wnt-beta-catenin
OT  - dental pulp stem cells
OT  - dentin dysplasia type I
OT  - odontoblastic differentiation
EDAT- 2019/06/21 06:00
MHDA- 2019/12/04 06:00
CRDT- 2019/06/21 06:00
PHST- 2019/06/21 06:00 [entrez]
PHST- 2019/06/21 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
AID - 10.1080/21691401.2019.1629950 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2575-2584. doi: 
      10.1080/21691401.2019.1629950.