PMID- 31219127
OWN - NLM
STAT- MEDLINE
DCOM- 20191219
LR  - 20191219
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 11
IP  - 26
DP  - 2019 Jul 14
TI  - Measurements of aptamer-protein binding kinetics using graphene field-effect 
      transistors.
PG  - 12573-12581
LID - 10.1039/c9nr02797a [doi]
AB  - Quantifying interactions between biomolecules subject to various environmental 
      conditions is essential for applications such as drug discovery and precision 
      medicine. This paper presents an investigation of the kinetics of environmentally 
      dependent biomolecular binding using an electrolyte-gated graphene field-effect 
      transistor (GFET) nanosensor. In this approach, biomolecular binding occurring on 
      and in the vicinity of a graphene surface induces a change in carrier 
      concentration, whose resulting conductance change is measured. This allows a 
      systematic study of the kinetic properties of the binding system. We apply this 
      approach to the specific binding of human immunoglobulin E (IgE), an antibody 
      involved in parasite immunity, with an aptamer at different ionic strengths 
      (Na(+) and Mg(2+)) and temperatures. Experimental results demonstrate 
      increased-rate binding kinetics at higher salt-ion concentrations and 
      temperatures. In particular, the divalent cation Mg(2+) yields more pronounced 
      changes in the conformational structure of the aptamer than the monovalent cation 
      Na(+). In addition, the dissociation of the aptamer-protein complex at room 
      temperature is found to be characterized by large unfavorable changes in the 
      activation enthalpy and entropy.
FAU - Wang, Xuejun
AU  - Wang X
AUID- ORCID: 0000-0002-7034-0460
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA. qlin@columbia.edu and State Key Laboratory of Molecular Engineering of 
      Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 
      200438, China.
FAU - Hao, Zhuang
AU  - Hao Z
AUID- ORCID: 0000-0003-4776-6022
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA. qlin@columbia.edu.
FAU - Olsen, Timothy R
AU  - Olsen TR
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA. qlin@columbia.edu.
FAU - Zhang, Wenjun
AU  - Zhang W
AD  - Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, S7N 
      5A9, Canada.
FAU - Lin, Qiao
AU  - Lin Q
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027, 
      USA. qlin@columbia.edu.
LA  - eng
PT  - Journal Article
DEP - 20190620
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - *Biosensing Techniques
MH  - Graphite/*chemistry
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Kinetics
MH  - *Transistors, Electronic
EDAT- 2019/06/21 06:00
MHDA- 2019/12/20 06:00
CRDT- 2019/06/21 06:00
PHST- 2019/06/21 06:00 [pubmed]
PHST- 2019/12/20 06:00 [medline]
PHST- 2019/06/21 06:00 [entrez]
AID - 10.1039/c9nr02797a [doi]
PST - ppublish
SO  - Nanoscale. 2019 Jul 14;11(26):12573-12581. doi: 10.1039/c9nr02797a. Epub 2019 Jun 
      20.