PMID- 31220552
OWN - NLM
STAT- MEDLINE
DCOM- 20200724
LR  - 20200724
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Linking)
VI  - 150
DP  - 2019 Aug
TI  - Calcitriol protects the Blood-Brain Barrier integrity against ischemic stroke and 
      reduces vasogenic brain edema via antioxidant and antiapoptotic actions in rats.
PG  - 281-289
LID - S0361-9230(19)30129-7 [pii]
LID - 10.1016/j.brainresbull.2019.06.010 [doi]
AB  - BACKGROUND: Vasogenic brain edema is the most important complication of ischemic 
      stroke that aggravates primary brain injury. Ischemia-Reperfusion (IR)-induced 
      Blood-Brain Barrier (BBB) impairment limits the use of recombinant tissue 
      plasminogen activator (r-tPA) by increasing the possibility of hemorrhagic 
      transformation and contributing to vasogenic edema and neuroinflammation. This 
      study examined the effects of post-ischemic treatment with calcitriol on cerebral 
      infarction, vasogenic edema formation and BBB disruption in a rat model of 
      ischemic stroke. METHODS: Male Sprague-Dawley rats were divided into three main 
      groups, including the sham, IR + vehicle and IR + calcitriol groups. Transient 
      focal cerebral ischemia was induced by a 60-min-long occlusion of the left middle 
      cerebral artery. The infarct volume, brain edema, BBB permeability and 
      antioxidant enzyme activities were evaluated 24 h after ischemia. 
      Immunohistochemical analysis was conducted to investigate cell apoptosis and 
      Brain-Derived Neurotrophic Factor (BDNF) protein expression five days after 
      ischemia. RESULTS: Compared to the IR + vehicle group, the IR + calcitriol group 
      showed a reduced brain infarction volume, attenuated brain edema formation and 
      improved BBB function. These protective effects were followed by the upregulation 
      of antioxidant enzyme activities in the brain tissue. Additionally, a diminished 
      cell apoptosis and an increased BDNF immunoreactivity were obtained in the 
      IR + calcitriol group. CONCLUSION: Calcitriol may reduce brain injury and 
      attenuate vasogenic edema by upregulating antioxidant enzymes activities, 
      reducing cell apoptosis and increasing BDNF protein in the brain tissue in a rat 
      model of ischemic stroke.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Sadeghian, Nooshin
AU  - Sadeghian N
AD  - Department of Physiology, Medical School, Ardabil University of Medical Sciences, 
      Ardabil, Iran.
FAU - Shadman, Javad
AU  - Shadman J
AD  - Department of Physiology, Medical School, Ardabil University of Medical Sciences, 
      Ardabil, Iran.
FAU - Moradi, Alireza
AU  - Moradi A
AD  - Department of Physiology, Medical School, Ardabil University of Medical Sciences, 
      Ardabil, Iran.
FAU - Ghasem Golmohammadi, Mohammad
AU  - Ghasem Golmohammadi M
AD  - Department of Anatomy, Medical School, Ardabil University of Medical Sciences, 
      Ardabil, Iran.
FAU - Panahpour, Hamdollah
AU  - Panahpour H
AD  - Department of Physiology, Medical School, Ardabil University of Medical Sciences, 
      Ardabil, Iran; Physiological Studies Research Center, Ardabil University of 
      Medical Sciences, Ardabil, Iran. Electronic address: panahpour.h@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190618
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Antioxidants)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Blood-Brain Barrier/*drug effects
MH  - Brain/metabolism
MH  - Brain Edema/*drug therapy
MH  - Brain Ischemia/metabolism
MH  - Calcitriol/metabolism/*pharmacology
MH  - Disease Models, Animal
MH  - Infarction, Middle Cerebral Artery/complications
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stroke/metabolism
MH  - Tissue Plasminogen Activator/pharmacology
OTO - NOTNLM
OT  - Antioxidant enzymes
OT  - Apoptosis
OT  - BDNF
OT  - Brain edema
OT  - Calcitriol
OT  - Stroke
EDAT- 2019/06/21 06:00
MHDA- 2020/07/25 06:00
CRDT- 2019/06/21 06:00
PHST- 2019/02/12 00:00 [received]
PHST- 2019/06/11 00:00 [revised]
PHST- 2019/06/12 00:00 [accepted]
PHST- 2019/06/21 06:00 [pubmed]
PHST- 2020/07/25 06:00 [medline]
PHST- 2019/06/21 06:00 [entrez]
AID - S0361-9230(19)30129-7 [pii]
AID - 10.1016/j.brainresbull.2019.06.010 [doi]
PST - ppublish
SO  - Brain Res Bull. 2019 Aug;150:281-289. doi: 10.1016/j.brainresbull.2019.06.010. 
      Epub 2019 Jun 18.