PMID- 31220794
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR  - 20200930
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 216
DP  - 2019 Sep
TI  - Engineering a platform for nerve regeneration with direct application to nerve 
      repair technology.
PG  - 119263
LID - S0142-9612(19)30362-X [pii]
LID - 10.1016/j.biomaterials.2019.119263 [doi]
AB  - The development of effective treatment options for repair of peripheral nerves is 
      complicated by lack of knowledge concerning the interactions between cells and 
      implants. A promising device, the multichannel scaffold, incorporates microporous 
      channels, aligning glia and directing axonal growth across a nerve gap. To 
      enhance clinical outcomes of nerve repair, a platform, representative of current 
      implant technology, was engineered which 1) recapitulated key device features 
      (porosity and linearity) and 2) demonstrated remyelination of adult neurons. The 
      in vitro platform began with the study of Schwann cells on porous 
      polycaprolactone (PCL) and poly(lactide co-glycolide) (PLGA) substrates. Surface 
      roughness determined glial cell attachment, and an additional layer of 
      topography, 40 mum linear features, aligned Schwann cells and axons. In addition, 
      direct co-culture of sensory neurons with Schwann cells significantly increased 
      neurite outgrowth, compared to neurons cultured alone (naive or pre-conditioned). 
      In contrast to the control substrate (glass), on porous PCL substrates, Schwann 
      cells differentiated into a mature myelinating phenotype, expressing Oct-6, MPZ 
      and MBP. The direct applicability of this platform to nerve implants, including 
      its response to physiological cues, allows for optimization of cell-material 
      interactions, close observation of the regeneration process, and the study of 
      therapeutics, necessary to advance peripheral nerve repair technology.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Pawelec, K M
AU  - Pawelec KM
AD  - University of Michigan, Department of Mechanical Engineering, Ann Arbor, MI, 
      48109, USA.
FAU - Yoon, C
AU  - Yoon C
AD  - University of Michigan, Department of Cell and Developmental Biology, Ann Arbor, 
      MI, 48109, USA.
FAU - Giger, R J
AU  - Giger RJ
AD  - University of Michigan, Department of Cell and Developmental Biology, Ann Arbor, 
      MI, 48109, USA.
FAU - Sakamoto, J
AU  - Sakamoto J
AD  - University of Michigan, Department of Mechanical Engineering, Ann Arbor, MI, 
      48109, USA. Electronic address: jeffsaka@umich.edu.
LA  - eng
GR  - R01 EB014986/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190612
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Polyesters)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 24980-41-4 (polycaprolactone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Guided Tissue Regeneration
MH  - Mice, Inbred C57BL
MH  - *Nerve Regeneration
MH  - Neuroglia/*cytology/physiology
MH  - Neurons/*cytology/physiology
MH  - Polyesters/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry
MH  - Porosity
MH  - Schwann Cells/cytology/physiology
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Co-culture system
OT  - In vitro model
OT  - Myelination
OT  - Nerve repair
OT  - Peripheral nervous system
EDAT- 2019/06/21 06:00
MHDA- 2020/10/02 06:00
CRDT- 2019/06/21 06:00
PHST- 2019/01/31 00:00 [received]
PHST- 2019/06/05 00:00 [revised]
PHST- 2019/06/07 00:00 [accepted]
PHST- 2019/06/21 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/06/21 06:00 [entrez]
AID - S0142-9612(19)30362-X [pii]
AID - 10.1016/j.biomaterials.2019.119263 [doi]
PST - ppublish
SO  - Biomaterials. 2019 Sep;216:119263. doi: 10.1016/j.biomaterials.2019.119263. Epub 
      2019 Jun 12.