PMID- 31223024
OWN - NLM
STAT- MEDLINE
DCOM- 20190829
LR  - 20190829
IS  - 0006-9248 (Print)
IS  - 0006-9248 (Linking)
VI  - 120
IP  - 6
DP  - 2019
TI  - Evaluation of the repair of diaphyseal fracture of femoral bone using bone marrow 
      mesenchymal stem cells in nicotine-bearing rat.
PG  - 434-442
LID - 10.4149/BLL_2019_070 [doi]
AB  - OBJECTIVE: Following bone fractures during accidents, some patients suffer from 
      poor repair of bone fractures and subsequent aesthetic and psychological 
      problems. One of the treatments is based on transplantation of stem cells (seeded 
      on scaffolds) to the lesion site. Bone marrow stromal cells (BMSCs) are 
      multivalent stem cells which are able to reproduce and differentiate into 
      osteogenic cells. The objective of this study was to evaluate the treatment of 
      bone fractures by means of transplantation of the latter cells in rats. METHODS: 
      In this study, the therapeutic effect of mesenchymal stem cells from bone marrow 
      adipocytes was evaluated in bone fractures. BMSCs were isolated from rat femur. 
      Two sources of differentiated and non-differentiated osteocyte cells were 
      provided and mixed with collagen in order to be transferred to animals divided in 
      three main groups of model: nicotine, non-nicotine and control groups. After four 
      weeks, the repair of the fracture that had been inflicted by a 2‑mm drill into 
      the diaphyseal region of the femoral bone was investigated by radiographic tests 
      and histopathologic staining. RESULTS: The radiographic results as well as those 
      of histopathologic staining showed that osteogenesis was more intensive in the 
      non-nicotine group than in the nicotine group with differentiated and 
      non-differentiated osteocyte cells. CONCLUSION: The transplantation of 
      differentiated BMSCs to a bone lesion affected the repair of bone fractures while 
      the nicotine agent played an important role in delaying the bone regeneration 
      (Tab. 1, Fig. 8, Ref. 31).
FAU - Riahi, M
AU  - Riahi M
FAU - Parivar, K
AU  - Parivar K
FAU - Baharara, J
AU  - Baharara J
FAU - Zandi, R
AU  - Zandi R
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Bratisl Lek Listy
JT  - Bratislavske lekarske listy
JID - 0065324
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Femur
MH  - *Fractures, Bone/therapy
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Nicotine
MH  - Osteogenesis
MH  - Rats
OTO - NOTNLM
OT  - bone marrow mesenchymal stem cell
OT  - nicotine
OT  - rat bone fracture regeneration.
EDAT- 2019/06/22 06:00
MHDA- 2019/08/30 06:00
CRDT- 2019/06/22 06:00
PHST- 2019/06/22 06:00 [entrez]
PHST- 2019/06/22 06:00 [pubmed]
PHST- 2019/08/30 06:00 [medline]
AID - 10.4149/BLL_2019_070 [doi]
PST - ppublish
SO  - Bratisl Lek Listy. 2019;120(6):434-442. doi: 10.4149/BLL_2019_070.