PMID- 31223106
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20190906
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 35
IP  - 5
DP  - 2019 May
TI  - [The 810 nm low-level laser inhibits the polarization of M1 bone marrow-derived 
      macrophages to promote neuronal axon growth of dorsal root ganglion].
PG  - 385-392
AB  - Objective To investigate the effect of low-level laser on the polarization and 
      secretory phenotype of primary cultured M1 bone marrow-derived macrophages 
      (BMDMs) in neuronal axons of dorsal root ganglion (DRG). Methods BMDMs were 
      isolated and cultured, and lipopolysaccharide (LPS) combined with IFN-gamma were used 
      to induce M1 phenotype polarization of BMDMs, and then F4/80 and CD16/32 
      expression was detected by flow cytometry. The mature M1 type BMDMs were randomly 
      divided into low-level laser group and control group. The laser exposure group 
      was subjected to the laser treatments of 0.4J, 4J and 10J, and no laser was used 
      in the control group. After 24 hours of laser exposure, the mRNA level of 
      inducible nitric oxide synthase (iNOS) of M1 type BMDMs was detected by reverse 
      transcription PCR, and the protein level of iNOS was detected by Western blot 
      analysis. The levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta 
      (IL-1beta), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) 
      in the supernatant of cultured cells were tested by ELISA. DRG neurons were 
      cultured with the supernatant fluid of M1 type BMDMs, and immunofluorescence 
      cytochemistry was employed to detect neuronal nuclei (NeuN) and beta-tubulin III 
      expression of DRG neurons for determining the influence on the growth of DRG 
      neuronal axons. Results Compared with the control group, the mRNA level of iNOS 
      in M1 type BMDMs dramatically increased after 24 hours of low-level laser 
      exposure. Among the 3 groups with different energy levels, the decrease of iNOS 
      mRNA level was the most obvious in the group with 4J laser exposure. The protein 
      levels of iNOS in the groups with 0.4J- and 4J- laser exposure were reduced more 
      significantly than that in the control group, and the down-regulation was more 
      prominent in the group with 4J laser exposure than that with 0.4J laser exposure. 
      In addition, the secretion of TNF-alpha from M1 type BMDMs was reduced more 
      significantly in the groups of 4J- and 10J- laser exposure than that in the 
      control group. With regard to IL-1beta, its secretion was inhibited in all the laser 
      exposure groups compared with the control group, and the suppression was more 
      prominent in the groups of 0.4J- and 4J-laser exposure than that in the group of 
      10J-laser exposure. Furthermore, 4J-laser exposure significantly potentiated the 
      secretion of BDNF and NGF in M1 type BMDMs compared with the control group. 
      Moreover, co-culture with the supernatants from 4J- and 10J-laser exposure groups 
      could significantly promote the growth of axons of DRG neurons. Conclusion 
      Low-level laser exposure can inhibit the polarization of M1 type BMDM and the 
      secretion of pro-inflammatory factor including TNF-alpha and IL-1beta. Besides, 
      low-level laser exposure could contribute to the secretion of neurotrophic 
      factors including BDNF and NGF, and promote the growth of DRG axon, and this 
      effect is dose-dependent.
FAU - Dai, Chen
AU  - Dai C
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China.
FAU - Sun, Jiakai
AU  - Sun J
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China.
FAU - Zhang, Jiawei
AU  - Zhang J
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China.
FAU - Liang, Zhuowen
AU  - Liang Z
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China.
FAU - Wang, Zhe
AU  - Wang Z
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China.
FAU - Hu, Xueyu
AU  - Hu X
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China. 
      *Corresponding authors, E-mail: huxueyu@fmmu.edu.cn.
FAU - Luo, Zhuojing
AU  - Luo Z
AD  - Xijing Orthopaedics Hospital, Air Force Medical University, Xi'an 710032, China. 
      *Corresponding authors, E-mail: luozhuojing@fmmu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (IL1B protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NGF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Axons/*physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - *Cell Polarity
MH  - Cells, Cultured
MH  - Ganglia, Spinal/*growth & development
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - *Lasers
MH  - Lipopolysaccharides
MH  - Macrophages/*cytology/radiation effects
MH  - Nerve Growth Factor/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2019/06/22 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/22 06:00
PHST- 2019/06/22 06:00 [entrez]
PHST- 2019/06/22 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 May;35(5):385-392.